Detection of Saint Louis Encephalitis Virus in Dengue-Suspected Cases During a Dengue 3 Outbreak

被引：41
作者：
Bernardes Terzian, Ana Carolina [1 ]
Mondini, Adriano [1 ]
de Moraes Bronzoni, Roberta Vieira [1 ]
Drumond, Betania Paiva [2 ]
Ferro, Bianca Piovezan [1 ]
Sotello Cabrera, Eliana Marcia [3 ]
Moraes Figueiredo, Luis Tadeu [4 ]
Chiaravalloti-Neto, Francisco [5 ]
Nogueira, Mauricio Lacerda [1 ]
机构：
[1] Fac Med Sao Jose do Rio Preto FAMERP, Lab Pesquisa Virol, BR-15090000 Sao Jose Do Rio Preto, SP, Brazil
[2] FIOCRUZ MG, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil
[3] Fac Med Sao Jose do Rio Preto FAMERP, Dept Saude Colet, BR-15090000 Sao Jose Do Rio Preto, SP, Brazil
[4] Fac Med Ribeirao Preto, Ctr Pesquisa Virol, Ribeirao Preto, Brazil
[5] Superintendencia Controle Endemias SUCEN, Sao Jose Do Rio Preto, Brazil
来源：
VECTOR-BORNE AND ZOONOTIC DISEASES | 2011年 / 11卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
Acute febrile illness; Arbovirus; Dengue virus; Molecular diagnostic; SLEV envelope gene; GENETIC-VARIATION; NESTED PCR; INFECTION; IDENTIFICATION; ALPHAVIRUSES; STRAINS; DENV-3; ASSAYS;
D O I：
10.1089/vbz.2009.0200
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Arboviruses are frequently associated with outbreaks in humans and represent a serious public health problem. Among the Brazilian arboviruses, Mayaro virus, Dengue virus (DENV), Yellow Fever virus, Rocio virus, Saint Louis Encephalitis virus (SLEV), and Oropouche virus are responsible for most of human cases. All these arboviruses usually produce undistinguishable acute febrile illness, especially in the acute phase of infection. In this study we investigated the presence of arboviruses in sera of 519 patients presenting acute febrile illness, during a dengue outbreak in Sao Jose do Rio Preto City (Sao Paulo, Brazil). A multiplex-nested RT-polymerase chain reaction assay was applied to detect and identify the main Brazilian arboviruses (Flavivirus, Alphavirus, and Orthobunyavirus genera). The molecular analysis showed that 365 samples were positive to DENV-3, 5 to DENV-2, and 8 to SLEV. Among the positive samples, one coinfection was detected between DENV-2 and DENV-3. The phylogenetic analysis of the SLEV envelope gene indicated that the virus circulating in city is related to lineage V strains. These results indicated that during that large DENV-3 outbreak in 2006, different arboviruses cocirculated causing human disease. Thus, it is necessary to have an efficient surveillance system to control the dissemination of these arboviruses in the population.
引用
收藏
页码：291 / 300
页数：10
相关论文
共 54 条
  • [1] Evolution and dispersal of St. Louis encephalitis virus in the Americas
    Auguste, Albert J.
    Pybus, Oliver G.
    Carrington, Christine V. F.
    [J]. INFECTION GENETICS AND EVOLUTION, 2009, 9 (04) : 709 - 715
  • [2] Phylogenetic and evolutionary analyses of St. Louis encephalitis virus genomes
    Baillie, Gregory J.
    Kolokotronis, Sergios-Orestis
    Waltari, Eric
    Maffei, Joseph G.
    Kramer, Laura D.
    Perkins, Susan L.
    [J]. MOLECULAR PHYLOGENETICS AND EVOLUTION, 2008, 47 (02) : 717 - 728
  • [3] Concurrent infections by all four dengue virus serotypes during an outbreak of dengue in 2006 in Delhi, India
    Bharaj, Preeti
    Chahar, Harendra S.
    Pandey, Anubhav
    Diddi, Kavita
    Dar, Lalit
    Guleria, Randeep
    Kabra, Sushil K.
    Broor, Shobha
    [J]. VIROLOGY JOURNAL, 2008, 5 (1)
  • [4] False-positive results and contamination in nucleic acid amplification assays: Suggestions for a prevent and destroy strategy
    Borst, A
    Box, ATA
    Fluit, AC
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2004, 23 (04) : 289 - 299
  • [5] *BRAZ MIN HLTH, 2009, INF EP
  • [6] *BRAZ MIN HLTH, 2009, DENG BRAS INF EP
  • [7] *BRAZ MIN HLTH, 2008, INF EP DENG
  • [8] *BRAZ MIN HLTH, 2007, INF EP DENG
  • [9] *BRAZ MIN HLTH, 2005, SECR VIG SAUD GUIA V
  • [10] Duplex reverse transcription-PCR followed by nested PCR assays for detection and identification of Brazilian Alphaviruses and Flaviviruses
    Bronzoni, RVD
    Baleotti, FG
    Nogueira, RMR
    Nunes, M
    Figueiredo, LTM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (02) : 696 - 702
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