Computer-assisted simulations of phosphofructokinase-1 kinetics using simplified velocity equations

Equations useful for simulating the kinetic behavior of phosphofructokinase are presented. The equations, which are based on the concerted transition (symmetry) model for allosteric enzymes, account for substrate inhibition by MgATP. cooperative binding by F-6-P, activation by F-2,6-P-2, and deinhibition by AMP. Velocity calculations can be performed using either a spreadsheet program (e.g., MS Excel) or a web-based program (e.g.. Authorware). Both approaches are illustrated. (C) 2001 IUBMB. Published by Elsevier Science Ltd. All rights reserved.