Liposomes and blood cells: A flow cytometric study

被引:18
|
作者
Constantinescu, I [1 ]
Levin, E [1 ]
Gyongyossy-Issa, M [1 ]
机构
[1] Univ British Columbia, Dept Pathol, Canadian Blood Serv Res & Dev, Vancouver, BC V6T 2B5, Canada
来源
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2003年 / 31卷 / 04期
关键词
liposomes; blood cells; erythrocytes; leukocytes; platelets; fusion; POLYETHYLENE-GLYCOL-LIPOSOMES; IN-VIVO; CIRCULATION TIME; MEMBRANE-PROTEINS; FUSION; CLEARANCE; PLATELETS; SIZE; FORMULATIONS; COMPLEMENT;
D O I
10.1081/BIO-120025410
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To clarify the interactions of liposomes with blood cells, this study examined the behaviour of liposomes of a range of compositions in the presence of purified human blood cells in buffer or plasma; or in whole blood, or in mice in vivo. Liposomes, labeled with the hydrophilic fluorochrome, carboxy fluorescein (CF), or with membrane-sequestering R 18 or FITC-labeled phospholipids, were mixed with blood cells and the appearance of the fluorochromes in the blood cell population was monitored by flow cytometry. Irrespective of composition, with or without poly(ethylene glycol), all types of liposomes were found to interact rapidly and dose-dependently with red cells, leukocytes and platelets, both in vitro and in vivo. This took place equally in the presence and the absence of plasma proteins and functional enzyme cascades, suggesting that the prime facie interaction is opsonization-independent and is consistent with liposome-blood cell fusion.
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页码:395 / 424
页数:30
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