Anti-inflammatory effects of isoalantolactone on LPS-stimulated BV2 microglia cells through activating GSK-3β-Nrf2 signaling pathway

被引：25

作者：

Wang, Min ^{[1
]}

Wang, Kai ^{[2
]}

Gao, Xiaoping ^{[1
]}

Zhao, Kai ^{[1
]}

Chen, Hemu ^{[1
]}

Xu, Mei ^{[1
]}

机构：

[1] Anhui Med Univ, Affiliated Hosp 1, Dept Rehabil Med, Hefei 230022, Anhui, Peoples R China

[2] Anhui Med Univ, Affiliated Hosp 1, Dept Neurol, Hefei 230022, Anhui, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2018年 / 65卷

关键词：

Isoalantolactone; LPS; TNF-alpha; Nrf2; NF-kappa B; NF-KAPPA-B; INFLAMMATORY RESPONSE; NEURODEGENERATION; ANTIOXIDANT; MACROPHAGES; INHIBITION; CYTOKINES; DISEASE; MODEL;

D O I：

10.1016/j.intimp.2018.10.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Isoalantolactone (ISO) is a sesquiterpene lactone isolated from Inula helenium that has been known to exhibit anti-inflammatory effect. Nevertheless, the effects of ISO on neuroinflammation have not been explored. BV2 microglia cells were pretreated with ISO and then stimulated with LPS. ISO attenuated the production of inflammatory mediators, such as TNF-alpha, IL-beta, NO, and PGE(2) in LPS-stimulated BV2 microglia cells. ISO suppressed the LPS-induced NF-kappa B activation in a concentration-dependent manner. The expression of Nrf2 and HO-1 were increased by the treatment of ISO. Inhibition of Nrf2 by siRNA could reverse the anti-inflammatory effects of ISO, as confirmed by the reversed inflammatory mediator production. Furthermore, ISO increased the level of phosphorylated GSK-3 beta, the upstream molecule of Nrf2. In conclusion, these results indicated that ISO might exhibit its anti-inflammatory effects through activating GSK-3 beta-Nrf2 signaling pathway.

引用

页码：323 / 327

页数：5

共 25 条

[21] NF-κB:: a key role in inflammatory diseases [J].

Tak, PP ;

Firestein, GS .

JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (01) :7-11

[22] Activation of liver X receptors inhibit LPS-induced inflammatory response in primary bovine mammary epithelial cells [J].

Wang, Jingjing ;

Xiao, Chong ;

Wei, Zhengkai ;

Wang, Yanan ;

Zhang, Xu ;

Fu, Yunhe .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2018, 197 :87-92

[23] Morin suppresses inflammatory cytokine expression by downregulation of nuclear factor-κB and mitogen-activated protein kinase (MAPK) signaling pathways in lipopolysaccharide-stimulated primary bovine mammary epithelial cells [J].

Wang, Jingjing ;

Guo, Changming ;

Wei, Zhengkai ;

He, Xuexiu ;

Kou, Jinhua ;

Zhou, Ershun ;

Yang, Zhengtao ;

Fu, Yunhe .

JOURNAL OF DAIRY SCIENCE, 2016, 99 (04) :3016-3022

[24] Therapeutic potential of inhibition of the NF-κB pathway in the treatment of inflammation and cancer [J].

Yamamoto, Y ;

Gaynor, RB .

JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (02) :135-142

[25] Involvement of Pro- and Anti-Inflammatory Cytokines and Chemokines in the Pathophysiology of Traumatic Brain Injury [J].

Ziebell, Jenna M. ;

Morganti-Kossmann, Maria Cristina .

NEUROTHERAPEUTICS, 2010, 7 (01) :22-30

← 1 2 3 →