2D FT-ICR MS of Calmodulin: A Top-Down and Bottom-Up Approach

被引:34
|
作者
Floris, Federico [1 ]
van Agthoven, Maria [1 ]
Chiron, Lionel [2 ]
Soulby, Andrew J. [1 ]
Wootton, Christopher A. [1 ]
Lam, Yuko P. Y. [1 ]
Barrow, Mark P. [1 ]
Delsuc, Marc-Andre [2 ,3 ]
O'Connor, Peter B. [1 ]
机构
[1] Univ Warwick, Coventry, W Midlands, England
[2] CASC4DE, Illkirch Graffenstaden, France
[3] IGBMC, Illkirch Graffenstaden, France
基金
英国工程与自然科学研究理事会;
关键词
Tandem mass spectrometry; FTICR mass spectrometry; 2-Dimensional mass spectrometry; ION-CYCLOTRON RESONANCE; INFRARED MULTIPHOTON DISSOCIATION; MASS-SPECTROMETRY; FRAGMENTATION PATHWAYS; NOISE; PEPTIDES;
D O I
10.1007/s13361-016-1431-z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry (2D FT-ICR MS) allows data-independent fragmentation of all ions in a sample and correlation of fragment ions to their precursors through the modulation of precursor ion cyclotron radii prior to fragmentation. Previous results show that implementation of 2D FT-ICR MS with infrared multi-photon dissociation (IRMPD) and electron capture dissociation (ECD) has turned this method into a useful analytical tool. In this work, IRMPD tandem mass spectrometry of calmodulin (CaM) has been performed both in one-dimensional and two-dimensional FT-ICR MS using a top-down and bottom-up approach. 2D IRMPD FT-ICR MS is used to achieve extensive inter-residue bond cleavage and assignment for CaM, using its unique features for fragment identification in a less time- and sample-consuming experiment than doing the same thing using sequential MS/MS experiments.
引用
收藏
页码:1531 / 1538
页数:8
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