In situ proteolysis for protein crystallization and structure determination

被引：151
|
作者：
Dong, Aiping
Xu, Xiaohui
Edward, Aled M.
机构：
[1] Univ Toronto, Struct Genom Consortium, Toronto, ON M5G 1L5, Canada
[2] Univ Toronto, Midwest Ctr Struct Genom, Toronto, ON M5G 1L6, Canada
来源：
NATURE METHODS | 2007年 / 4卷 / 12期
基金：
英国惠康基金;
关键词：
D O I：
10.1038/nmeth1118
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
引用
收藏
页码：1019 / 1021
页数：3
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