Sensitization to Fas-mediated apoptosis by dengue virus capsid protein

被引：46

作者：

Limjindaporn, Thawornchai ^{[1
]}

Netsawang, Janjuree

Noisakran, Sansanee

Thiemmeca, Somchai

Wongwiwat, Wiyada

Sudsaward, Sangkab

Avirutnan, Panisadee

Puttikhunt, Chunya

Kasinrerk, Watchara

Sriburi, Rungtawan

Sittisombut, Nopporn

Yenchitsomanus, Pa-Thai

Maiasit, Prida

机构：

[1] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Anat, Bangkok 10700, Thailand

[2] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Immunol, Bangkok 10700, Thailand

[3] Mahidol Univ, Siriraj Hosp, Fac Med, Med Mol Biol Unit, Bangkok 10700, Thailand

[4] Chiang Mai Univ, Fac Assoc Med Sci, Dept Clin Immunol, Chiang Mai 50000, Thailand

[5] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Med Biotechnol Unit, Bangkok, Thailand

[6] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai 50000, Thailand

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 362卷 / 02期

关键词：

dengue virus capsid protein; human Daxx; protein interaction; apoptosis;

D O I：

10.1016/j.bbrc.2007.07.194

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dengue fever (DF) and dengue hemorrhagic fever (DHF) are important public health problems in tropical regions. Abnormal hemostasis and plasma leakage are the main patho-physiological changes in DHF. However, hepatomegaly, hepatocellular necrosis and fulminant hepatic failure are occasionally observed in patients with DHF. Dengue virus-infected liver cells undergo apoptosis but the underlying molecular mechanism remains unclear. Using a yeast two-hybrid screen, we found that dengue virus capsid protein (DENV Q physically interacts with the human death domain-associated protein Daxx, a Fas-associated protein. The interaction between DENV C and Daxx in dengue virus-infected liver cells was also demonstrated by co-immunoprecipitation and double immunofluorescence staining. The two proteins were predominantly co-localized in the cellular nuclei. Fas-mediated apoptotic activity in liver cells constitutively expressing DENV C was induced by anti-Fas antibody, indicating that the interaction of DENV C and Daxx involves in apoptosis of dengue virus-infected liver cells. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：334 / 339

页数：6

共 34 条

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