Differentiation of human neural progenitor cells regulated by Wnt-3a

被引：31
作者：
Huebner, Rayk [1 ]
Schmoele, Anne-Caroline [1 ]
Liedmann, Andrea [1 ]
Frech, Moritz J. [1 ]
Rolls, Arndt [1 ]
Luo, Jiankai [1 ]
机构：
[1] Univ Rostock, Sch Med, Albrecht Kossel Inst Neuroregenerat AKos, D-18147 Rostock, Germany
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 400卷 / 03期
关键词：
Wnt-3a; Wnt/beta-catenin signaling; Neural stem cells; STEM-CELLS; NEURONAL DIFFERENTIATION; EXPRESSION; PROLIFERATION; NEUROGENESIS; CANCER; FATE; GENE; TIME;
D O I：
10.1016/j.bbrc.2010.08.066
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Wnt ligands play pivotal roles in the control of cell growth and differentiation during central nervous system development via the Wnt signaling pathway In this study, we investigated the effects of Wnt-3a and beta-catenin on the differentiation of ReNcell VM human neural progenitor cells After overexpression of Wnt-3a or mutant-stabilized beta-catenin in ReNcell VM cells, their effects on TCF-mediated transcription, Wnt target gene expression and differentiation into neuronal and glial cells were investigated. Our results show that activation of Wnt/beta-catenin signaling increases TCF-mediated transcription and the expression of the Wnt target genes Axin2, LEF1 and CyclinD1 in ReNcell VM cells. In contrast to mutant-stabilized beta-catenin, Wnt-3a increases neurogenesis during the differentiation of ReNcell VM cells Thus, our data suggest that neurogenesis induced by Wnt-3a is independent of the transcriptional activity of Wnt/beta-catenin pathway in ReNcell VM cells. (C) 2010 Elsevier Inc All rights reserved
引用
收藏
页码：358 / 362
页数：5
相关论文
共 23 条
[1]   Defining a developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/progenitor cells [J].
Aiba, Kazuhiro ;
Sharov, Alexei A. ;
Carter, Mark G. ;
Foroni, Chiara ;
Vescovi, Angelo L. ;
Ko, Minoru S. H. .
STEM CELLS, 2006, 24 (04) :889-895
[2]   Regulation of cyclin D1 expression by mTORC1 signaling requires eukaryotic initiation factor 4E-binding protein 1 [J].
Averous, J. ;
Fonseca, B. D. ;
Proud, C. G. .
ONCOGENE, 2008, 27 (08) :1106-1113
[3]   Excess β-catenin promotes accumulation of transcriptionally active p53 [J].
Damalas, A ;
Ben-Ze'ev, A ;
Simcha, I ;
Shtutman, N ;
Leal, JFN ;
Zhurinsky, J ;
Geiger, B ;
Oren, M .
EMBO JOURNAL, 1999, 18 (11) :3054-3063
[4]   Differential development of neuronal physiological responsiveness in two human neural stem cell lines [J].
Donato, Roberta ;
Miljan, Erik A. ;
Hines, Susan J. ;
Aouabdi, Sihem ;
Pollock, Kenneth ;
Patel, Sara ;
Edwards, Frances A. ;
Sinden, John D. .
BMC NEUROSCIENCE, 2007, 8 (1)
[5]   The Wnt antagonist DICKKOPF-1 gene is a downstream target of β-catenin/TCF and is downregulated in human colon cancer [J].
Gonzálex-Sancho, JM ;
Aguilera, O ;
García, JM ;
Pendás-Franco, N ;
Peña, C ;
Cal, S ;
de Herreros, AG ;
Bonilla, F ;
Muñoz, A .
ONCOGENE, 2005, 24 (06) :1098-1103
[6]   Stage-dependent fate determination of neural precursor cells in mouse forebrain [J].
Hirabayashi, Y ;
Gotoh, Y .
NEUROSCIENCE RESEARCH, 2005, 51 (04) :331-336
[7]   Canonical Wnt signaling transiently stimulates proliferation and enhances neurogenesis in neonatal neural progenitor cultures [J].
Hirsch, Cordula ;
Campano, Louise M. ;
Woehrle, Simon ;
Hecht, Andreas .
EXPERIMENTAL CELL RESEARCH, 2007, 313 (03) :572-587
[8]   The presence of FGF2 signaling determines whether β-catenin exerts effects on proliferation or neuronal differentiation of neural stem cells [J].
Israsena, N ;
Hu, M ;
Fu, WM ;
Kan, LX ;
Kessler, JA .
DEVELOPMENTAL BIOLOGY, 2004, 268 (01) :220-231
[9]   Inhibition of canonical Wnt signaling promotes gliogenesis in P0-NSCs [J].
Kunke, David ;
Bryja, Vitezslav ;
Mygland, Line ;
Arenas, Ernest ;
Krauss, Stefan .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 386 (04) :628-633
[10]  
Lee SMK, 2000, DEVELOPMENT, V127, P457
123