Exploring a multiplex DNA methylation-based SNP typing method for body fluids identification: As a preliminary report
被引:13
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作者:
Xie, Bowen
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Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R China
Xie, Bowen
[1
]
Song, Feng
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Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R China
Song, Feng
[1
]
Wang, Shuangshuang
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Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R China
Wang, Shuangshuang
[1
]
Zhang, Ke
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Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R China
Zhang, Ke
[1
]
Li, Yingbi
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Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R China
Li, Yingbi
[1
]
Luo, Haibo
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Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R China
Luo, Haibo
[1
]
机构:
[1] Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Dept Forens Genet, 3-16 Renmin South Rd, Chengdu 610041, Peoples R China
DNA methylation;
Body fluid identification;
SNP;
SNaPshot;
BLOOD;
DIFFERENTIATION;
MIXTURES;
MARKERS;
SEMEN;
D O I:
10.1016/j.forsciint.2020.110329
中图分类号:
DF [法律];
D9 [法律];
R [医药、卫生];
学科分类号:
0301 ;
10 ;
摘要:
In forensic investigation, identification of the cellular origin from body fluid can be essential in the crime scene reconstruction. Recently, DNA methylation could potentially be used as a novel marker for body fluid identification. The simultaneous analysis of CpGs and neighboring single nucleotide polymorphisms (SNPs) has been proposed as an efficient assay for body fluids identification. In this study, a multiplex DNA methylation-based SNP typing system was developed. The specificity, sensitivity and detectability in mixtures and degraded samples were explored in our study. As results, four DNA methylation-based semen-specific SNP (SE1-4) showed good specificity, but two markers associative with saliva (SA1) and vaginal fluid (VA3) was observed cross-reactivity sporadically. Interesting, VA3 were found only presented in the female which may be useful for sexual identification. Moreover, this multiplex system successfully amplification in mixtures and aged samples which proves it be used as a valuable protocol in the identification of actual forensic samples. The strategy indicated that the approach was suitable and reliable for the body fluids analysis in mix stains in Han Chinese for forensic purposes. (C) 2020 Elsevier B.V. All rights reserved.
机构:
Seoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South KoreaSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Lee, Ji Eun
Lee, Jeong Min
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Seoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South KoreaSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Lee, Jeong Min
Naue, Jana
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机构:
Univ Freiburg, Med Ctr, Fac Med, Inst Forens Med, Freiburg, GermanySeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Naue, Jana
Fleckhaus, Jan
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机构:
Univ Cologne, Fac Med, Inst Legal Med, Cologne, Germany
Univ Cologne, Univ Hosp Cologne, Cologne, GermanySeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Fleckhaus, Jan
Freire-Aradas, Ana
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Univ Santiago de Compostela, Inst Forens Sci, Forens Genet Unit, Santiago De Compostela, Galicia, SpainSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Freire-Aradas, Ana
Neubauer, Jacqueline
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Univ Zurich, Zurich Inst Forens Med, Zurich, SwitzerlandSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Neubauer, Jacqueline
Pospiech, Ewelina
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机构:
Jagiellonian Univ, Malopolska Ctr Biotechnol, Krakow, PolandSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Pospiech, Ewelina
McCord, Bruce
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Florida Int Univ, Dept Chem & Biochem, Miami, FL 33199 USASeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
McCord, Bruce
Kalamara, Vivian
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Univ Med Ctr Rotterdam, Dept Genet Identificat, Erasmus MC, Rotterdam, NetherlandsSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Kalamara, Vivian
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Gauthier, Quentin
Mills, Carly
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George Washington Univ, Dept Forens Sci, Washington, DC 20052 USASeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Mills, Carly
Cao, Yijian
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George Washington Univ, Dept Forens Sci, Washington, DC 20052 USASeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Cao, Yijian
Wang, Zheng
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Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Inst Forens Med, Chengdu, Peoples R ChinaSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Wang, Zheng
Oh, Yu Na
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Minist Natl Def, Def Inst Forens Sci, Seoul, South KoreaSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Oh, Yu Na
Feng, Lei
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Minist Publ Secur, Inst Forens Sci, Beijing, Peoples R ChinaSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Feng, Lei
Schneider, Peter M.
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机构:
Univ Cologne, Fac Med, Inst Legal Med, Cologne, Germany
Univ Cologne, Univ Hosp Cologne, Cologne, GermanySeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Schneider, Peter M.
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Phillips, Christopher
Haas, Cordula
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Univ Zurich, Zurich Inst Forens Med, Zurich, SwitzerlandSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Haas, Cordula
Pisarek, Aleksandra
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Jagiellonian Univ, Malopolska Ctr Biotechnol, Krakow, PolandSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Pisarek, Aleksandra
Branicki, Wojciech
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Jagiellonian Univ, Inst Zool & Biomed Res, Lab Anthropol, Krakow, PolandSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Branicki, Wojciech
Podini, Daniele
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George Washington Univ, Dept Forens Sci, Washington, DC 20052 USASeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Podini, Daniele
Vidaki, Athina
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Univ Med Ctr Rotterdam, Dept Genet Identificat, Erasmus MC, Rotterdam, NetherlandsSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Vidaki, Athina
Tejero, Nicole Fernandez
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Florida Int Univ, Dept Chem & Biochem, Miami, FL 33199 USASeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Tejero, Nicole Fernandez
Ambroa-Conde, Adrian
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Univ Santiago de Compostela, Inst Forens Sci, Forens Genet Unit, Santiago De Compostela, Galicia, SpainSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Ambroa-Conde, Adrian
Mosquera-Miguel, Ana
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Univ Santiago de Compostela, Inst Forens Sci, Forens Genet Unit, Santiago De Compostela, Galicia, SpainSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Mosquera-Miguel, Ana
Lareu, Maria Victoria
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Univ Santiago de Compostela, Inst Forens Sci, Forens Genet Unit, Santiago De Compostela, Galicia, SpainSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Lareu, Maria Victoria
Hou, Yiping
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Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Inst Forens Med, Chengdu, Peoples R ChinaSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Hou, Yiping
Lee, Joo Young
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Minist Natl Def, Def Inst Forens Sci, Seoul, South KoreaSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
Lee, Joo Young
Lee, Hwan Young
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Seoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South KoreaSeoul Natl Univ, Dept Forens Med, Coll Med, 103 Daehak Ro, Seoul 03080, South Korea
机构:
Seoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South KoreaSeoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea
Kim, Bo Min
Park, Sang Un
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Seoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South KoreaSeoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea
Park, Sang Un
Schmelzer, Laura
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机构:
Univ Freiburg, Inst Forens Med, Med Ctr, Fac Med, Freiburg, GermanySeoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea
Schmelzer, Laura
Yang, Soo-Bin
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Seoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South KoreaSeoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea
Yang, Soo-Bin
Lee, Soong Deok
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Seoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea
Seoul Natl Univ, Coll Med, Inst Forens & Anthropol Sci, Seoul, South KoreaSeoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea
Lee, Soong Deok
Kim, Moon -Young
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Sungkyunkwan Univ, Dept Anat & Cell Biol, Lab Forens Med, Sch Med, Suwon, South KoreaSeoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea
Kim, Moon -Young
Naue, Jana
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机构:
Univ Freiburg, Inst Forens Med, Med Ctr, Fac Med, Freiburg, GermanySeoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea
Naue, Jana
Lee, Hwan Young
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机构:
Seoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea
Seoul Natl Univ, Coll Med, Inst Forens & Anthropol Sci, Seoul, South KoreaSeoul Natl Univ, Coll Med, Dept Forens Med, Seoul, South Korea