A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission

被引：35

作者：

Balestra, Aurelia C. ^{[1
]}

Zeeshan, Mohammad ^{[2
]}

Rea, Edward ^{[2
]}

Pasquarello, Carla ^{[1
]}

Brusini, Lorenzo ^{[1
,2
]}

Mourier, Tobias ^{[3
]}

Subudhi, Amit Kumar ^{[3
]}

Klages, Natacha ^{[1
]}

Arboit, Patrizia ^{[1
]}

Pandey, Rajan ^{[2
]}

Brady, Declan ^{[2
]}

Vaughan, Sue ^{[4
]}

Holder, Anthony A. ^{[5
]}

Pain, Arnab ^{[3
]}

Ferguson, David J. P. ^{[4
,6
]}

Hainard, Alexandre ^{[1
]}

Tewari, Rita ^{[2
]}

Brochet, Mathieu ^{[1
]}

机构：

[1] Univ Geneva, Fac Med, Geneva, Switzerland

[2] Univ Nottingham, Sch Life Sci, Nottingham, England

[3] King Abdullah Univ Sci & Technol, Biol & Environm Sci & Engn BESE Div, Thuwal, Saudi Arabia

[4] Oxford Brookes Univ, Dept Biol & Med Sci, Oxford, England

[5] Francis Crick Inst, Malaria Parasitol Lab, London, England

[6] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Lab Sci, Oxford, England

来源：

ELIFE | 2020年 / 9卷

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会; 瑞士国家科学基金会; 英国医学研究理事会;

关键词：

MULTIPLE SEQUENCE ALIGNMENT; CELL-CYCLE; BLOOD STAGES; CLUSTAL-W; PLASMODIUM; SELECTION; CDKS; IDENTIFICATION; PERFORMANCE; PHYLOGENIES;

D O I：

10.7554/eLife.56474

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cell cycle transitions are generally triggered by variation in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of divergent CDKs and cyclins of poorly understood function and interdependency. We show that Plasmodium berghei CDK-related kinase 5 (CRK5), is a critical regulator of atypical mitosis in the gametogony and is required for mosquito transmission. It phosphorylates canonical CDK motifs of components in the pre-replicative complex and is essential for DNA replication. During a replicative cycle, CRK5 stably interacts with a single Plasmodium-specific cyclin (SOC2), although we obtained no evidence of SOC2 cycling by transcription, translation or degradation. Our results provide evidence that during Plasmodium male gametogony, this divergent cyclin/CDK pair fills the functional space of other eukaryotic cell-cycle kinases controlling DNA replication.

引用

页码：1 / 25

页数：25

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