Regeneration of Periodontal Tissues in Non-human Primates with rhGDF-5 and Beta-Tricalcium Phosphate

被引:38
作者
Emerton, K. B. [1 ]
Drapeau, S. J. [1 ]
Prasad, H. [2 ]
Rohrer, M. [2 ]
Roffe, P. [3 ]
Hopper, K. [4 ]
Schoolfield, J. [5 ]
Jones, A. [5 ]
Cochran, D. L. [5 ]
机构
[1] Medtronic, Spinal & Biol Div, Memphis, TN 38132 USA
[2] Univ Minnesota, Hard Tissue Lab, Minneapolis, MN 55455 USA
[3] Medtron World Headquarters, Minneapolis, MN 55432 USA
[4] Mannheimer Fdn, Homestead, FL 33034 USA
[5] Univ Texas Hlth Sci Ctr San Antonio, Dept Periodont, San Antonio, TX 78229 USA
关键词
GDF-5; medical devices; periodontal regeneration; bone regeneration; biological factors; periodontal disease; RECOMBINANT HUMAN GROWTH; HUMAN GROWTH/DIFFERENTIATION FACTOR-5; SINUS FLOOR AUGMENTATION; GOETTINGEN MINIATURE PIG; ENAMEL MATRIX PROTEINS; DIFFERENTIATION FACTOR-5; AUTOGENOUS BONE; DEFECT MODEL; SUBSTITUTES; COMBINATION;
D O I
10.1177/0022034511423665
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The application of growth factors has been advocated in support of periodontal regeneration. Recombinant human growth and differentiation factor-5 (rhGDF-5), a member of the bone morphogenetic protein family, has been used to encourage periodontal tissue regeneration. This study evaluated the dose response of rhGDF-5 lyophilized onto beta-tricalcium phosphate (bTCP) granules for periodontal tissue regeneration in a baboon model. Periodontal defects were created bilaterally in 12 baboons by a split-mouth design. Plaque was allowed to accumulate around wire ligatures to create chronic disease. After 2 mos, the ligatures were removed, and a notch was placed at the base of the defect. Two teeth on each side of the mouth were randomly treated with bTCP only, 0.5, 1.0, or 2.0 mg rhGDF-5/g bTCP. Animals were sacrificed 5 mos post-treatment, with micro-CT and histomorphometric analysis performed. After 5 mos, analysis showed alveolar bone, cementum, and periodontal ligament formation in all treatment groups, with a dose-dependent increase in rhGDF-5-treated groups. Height of periodontal tissues also increased with the addition of rhGDF-5, and the amount of residual graft material decreased with rhGDF-5 treatment. Therefore, rhGDF-5 delivered on bTCP demonstrated effective regeneration of all 3 tissues critical for periodontal repair.
引用
收藏
页码:1416 / 1421
页数:6
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