Conformational Changes of Protein Adsorbed on Tailored Flat Substrates with Different Chemistries

被引:8
作者
Huang, He [1 ,2 ]
Xie, Jing [1 ,2 ]
Liu, Xiaoli [1 ,2 ]
Yuan, Lin [1 ]
Wang, Shasha [1 ]
Guo, Songxi [1 ,2 ]
Yu, Haoran [1 ]
Chen, Hong [1 ]
Zhang, Yanliang [3 ]
Wu, Xiaohu [3 ]
机构
[1] Soochow Univ, Jiangsu Key Lab Adv Funct Polymer Mat Design & Ap, Dept Polymer Sci & Engn, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Peoples R China
[2] Wuhan Univ Technol, Sch Mat Sci & Engn, Wuhan 430070, Peoples R China
[3] Thermo Fisher Sci Shanghai Co Ltd, Shanghai 201206, Peoples R China
基金
中国国家自然科学基金;
关键词
adsorption; monolayers; nanoparticles; proteins; Raman spectroscopy; SURFACE-ENHANCED RAMAN; REFLECTION/INFRARED CORRELATION SPECTROSCOPY; 2-DIMENSIONAL CORRELATION SPECTROSCOPY; TRANSFER RADICAL POLYMERIZATION; NEAR-INFRARED SPECTRA; EGG-WHITE LYSOZYME; SECONDARY STRUCTURE; AQUEOUS-SOLUTIONS; SILICA NANOPARTICLES; CIRCULAR-DICHROISM;
D O I
10.1002/cphc.201100398
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Changes in the bioactivity of a protein after being adsorbed on a material surface may result from conformational changes of the protein. Unfortunately, however, direct evidence of such conformational changes of proteins adsorbed on a flat material surface is sparse so far. This is because probing the conformation of an adsorbed protein on material surfaces, especially flat ones, remains a challenge due to considerable experimental difficulties. In this study, the surface-enhanced Raman scattering (SERS) technique is used to characterize the conformational changes of a protein (lysozyme) adsorbed on tailored flat gold substrates with different chemistries. Two such substrates are formed by self-assembly of octadecanethiol and thiolated PEG on gold chips (Au-C18 and Au-PEG). Preliminary results reveal that, compared to the hydrophobic Au-C18 surface, the hydrophilic Au-PEG surface has much smaller effect on the conformation of lysozyme in aqueous solution, which thereby keeps its high bioactivity. The conformational changes of lysozyme adsorbed on material surfaces with different chemistries are well correlated with changes in its bioactivity.
引用
收藏
页码:3642 / 3646
页数:5
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