Direct electrochemical regeneration of NADH from NAD+ using cholesterol-modified gold amalgam electrode

被引:43
作者
Baik, SH
Kang, C
Jeon, IC
Yun, SE [1 ]
机构
[1] Chonbuk Natl Univ, Inst Agr Sci & Technol, Dept Food Sci & Technol, Chonju 561756, South Korea
[2] Chonbuk Natl Univ, Dept Chem, Chonju 561756, South Korea
关键词
direct electrochemical; enzymatic reaction; modified electrode; NADH regeneration;
D O I
10.1023/A:1008865212773
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The efficiency of the direct electrochemical regeneration of NADH from NAD(+) was enhanced by applying a cholesterol-modified gold amalgam electrode. The modified electrode was prepared by immersing gold plate in mercury and casting few drops of cholesteryl oleate solution over the gold amalgam. Coenzymatically active NADH was formed from NAD(+) directly at the cholesterol-modified gold amalgam electrode which is supposed to hinder the dimerization of the NAD radicals on its membrane surface. The direct electrochemical NAD(+) reduction process was used favorably to drive an enzymatic reduction of pyruvate to D-lactate. D-Lactate of 18.2 mm was obtained from pyruvate of 25.3 mm at 21 h of total reaction time in the electrolysis of 50 cm(3) solution with the electrode of 6 cm(2)area. The turnover number for NAD(+) was estimated as 1400.
引用
收藏
页码:1 / 5
页数:5
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