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Discrimination of β-1,4-and β-1,3-Linkages in Native Oligosaccharides via Charge Transfer Dissociation Mass Spectrometry
被引:21
作者:
Buck-Wiese, Hagen
[5
,6
]
Fanuel, Mathieu
[3
,4
]
Liebeke, Manuel
[5
]
Hoang, Kim Le Mai
[7
]
Pardo-Vargas, Alonso
[7
]
Seeberger, Peter H.
[7
,8
]
Hehemann, Jan-Hendrik
[5
,6
]
Rogniaux, Helene
[3
]
Jackson, Glen P.
[1
,2
]
Ropartz, David
[3
,4
]
机构:
[1] West Virginia Univ, Dept Forens & Invest Sci, Morgantown, WV 26506 USA
[2] West Virginia Univ, C Eugene Bennett Dept Chem, Morgantown, WV 26506 USA
[3] INRAE, UR BIA, F-44316 Nantes, France
[4] INRAE, BIBS Facil, F-44316 Nantes, France
[5] Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany
[6] Marum Ctr Marine Environm Sci, Marine Glycobiol, D-28359 Bremen, Germany
[7] Max Planck Inst Colloids & Inteifaces, Dept Biomol Syst, D-14476 Potsdam, Germany
[8] Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany
基金:
美国国家科学基金会;
关键词:
ion/ion activation;
oligosaccharides;
structural characterization;
branching pattern determination;
isomers;
COLLISION-INDUCED DISSOCIATION;
BLOOD-GROUP;
SPECTRA;
ANTIGENS;
CATIONS;
D O I:
10.1021/jasms.0c00087
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
The connection between monosaccharides influences the structure, solubility, and biological function of carbohydrates. Although tandem mass spectrometry (MS/MS) often enables the compositional identification of carbohydrates, traditional MS/MS fragmentation methods fail to generate abundant cross-ring fragments of intrachain monosaccharides that could reveal carbohydrate connectivity. We examined the potential of helium-charge transfer dissociation (He-CTD) as a method of MS/MS to decipher the connectivity of beta-1,4- and beta-1,3-linked oligosaccharides. In contrast to collision-induced dissociation (CID), He-CTD of isolated oligosaccharide precursors produced both glycosidic and cross-ring cleavages of each monosaccharide. The radical-driven dissociation in He-CTD induced single cleavage events, without consecutive fragmentations, which facilitated structural interpretation. He-CTD of various standards up to a degree of polymerization of 7 showed that beta-1,4- and beta-1,3-linked carbohydrates can be distinguished based on diagnostic 3,5A fragment ions that are characteristic for beta-1,4-linkages. Overall, fragment ion spectra from He-CTD contained sufficient information to infer the connectivity specifically for each glycosidic bond. When testing He-CTD to resolve the order of beta-1,4- and beta-1,3-linkages in mixed-linked oligosaccharide standards, He-CTD spectra sometimes provided less confident assignment of connectivity. Ion mobility spectrometry-mass spectrometry (IMS-MS) of the standards indicated that ambiguity in the He-CTD spectra was caused by isobaric impurities in the mixed-linked oligosaccharides. Radical-driven dissociation induced by He-CTD can thus expand MS/MS to carbohydrate linkage analysis, as demonstrated by the comprehensive fragment ion spectra on native oligosaccharides. The determination of connectivity in true unknowns would benefit from the separation of isobaric precursors, through UPLC or IMS, before linkage determination via He-CTD.
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页码:1249 / 1259
页数:11
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