Quick detection of Listeria monocytogenes by means of real-time PCR

Molecular biological methods like the real-time PCR are well suited for a safe and rapid detection of Listeria and especially of Listeria monocytogenes. The use of commercially available kits in combination with adapted sample preparation is advantageous for the routine testing in the food industry. In addition to its high specificity and sensitivity, its superior reproducibility and short time to result, the described method is easy to use. Depending on the food matrix, the result is available after 24 h to 48 h. The procedure, consists of a short enrichment, a sample preparation to extract the DNA from the cells and the PCR. The data interpretation is carried out automatically and can be used directly for documentation purposes.