Distribution of cholesterol 24-hydroxylase in the monkey brain

被引：2

作者：

He, Xin ^{[1
]}

Ong, Wei-Yi ^{[3
]}

Hua, Qian ^{[2
]}

机构：

[1] Beijing Univ Chinese Med, Dept Anat, Beijing 100029, Peoples R China

[2] Beijing Univ Chinese Med, Sch Preclin Med, Beijing 100029, Peoples R China

[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Anat, Singapore 119260, Singapore

来源：

NEUROSCIENCE BULLETIN | 2010年 / 26卷 / 03期

基金：

中国国家自然科学基金;

关键词：

axon; cholesterol; 24-hydroxylase; oxysterols; HIPPOCAMPAL-NEURONS; ALZHEIMERS-DISEASE; AXONAL GROWTH; CDNA CLONING; OXYSTEROL; TURNOVER; HOMEOSTASIS; CELLS; 24S-HYDROXYCHOLESTEROL; LOCALIZATION;

D O I：

10.1007/s12264-010-1224-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Objective Cholesterol 24-hydroxylase catalyzes the conversion of cholesterol to 24-hydroxycholesterol, which is a major pathway for cholesterol elimination from the brain, since 24-hydroxycholesterol can readily cross the blood brain barrier. The present study aimed to elucidate the distribution of cholesterol 24-hydroxylase in the monkey brain. Methods The distribution of cholesterol 24-hydroxylase in the monkey brain was examined using Western blot and immunohistochemistry methods, and was observed under light microscopy and electron microscopy. Results High levels of cholesterol 24-hydroxylase were observed in projection neurons and neuropil in structures derived from telencephalon, including the cerebral neocortex, hippocampus, amygdala, nucleus basalis of Meynert, and striatum. Electron microscopy revealed that the enzyme was localized in the axon terminals. One the other hand, cholesterol 24-hydroxylase was expressed at a lower level in the thalamus, globus pallidus and brainstem. Conclusion The high level of cholesterol 24-hydroxylase in the telencephalon possibly reflects a high rate of cholesterol turnover in this part of brain.

引用

页码：197 / 204

页数：8

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