Plant diseases, such as the pinewood disease, PWD, have become a problem of economical and forestall huge proportions. These diseases, that are asymptomatic and characterized by a fast spread, have no cure developed to date. Besides, there are no technical means to diagnose the disease in situ, without causing tree damage, and help to assist the forest management. Herein is proposed a portable and non-damage system, based on electrical impedance spectroscopy, EIS, for biological applications. In fact, EIS has been proving efficacy and utility in wide range of areas. However, although commercial equipment is available, it is expensive and unfeasible for in vivo and in field applications. The developed EIS system is able to perform AC current or voltage scans, within a selectable frequency range, and its effectiveness in assessing pine decay was proven. The procedure and the results obtained for a population of 24 young pine trees (Pinus pinaster Aiton) are presented. Pine trees were kept in a controlled environment and were inoculated with the nematode (Bursaphelenchus xylophilus Nickle), that causes the PWD, and also with bark beetles (Tomicus destruens Wollaston). The obtained results may constitute a first innovative approach to the diagnosis of such types of diseases.