Sequential liquid-liquid extraction of D-amino acid oxidase from Trigonopsis variabilis

被引：0
作者：
Singh, P
Verma, N
Marwaha, SS
机构：
[1] Punjabi Univ, Dept Biotechnol, Patiala 147002, Punjab, India
[2] OSCST, Div Biotechnol, Chandigarh 160019, India
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 18期
关键词：
aqueous two-phase system; D-amino acid oxidase; Trigonopsis variabilis;
D O I：
10.1023/A:1011685430717
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A method for isolation of D-amino acid oxidase (DAAO) from disrupted Trigonopsis variabilis cells has been developed. In an aqueous two-phase system consisting of PEG6000 (220 g l(-1)), potassium phosphate (110 g l(-1), K2HPO4 + KH2PO4 = 10.1:1, mol mol(-1)) and dl-methionine (11 g l(-1)), the major portion of cellular proteins (87%) was partitioned into the salt phase. By sequential extraction, 48% of DAAO was recovered in PEG phase, giving a yield of 211 U mg protein(-1).
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页码：1479 / 1483
页数：5
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