In Situ Conjugation of Dithiophenol Maleimide Polymers and Oxytocin for Stable and Reversible Polymer-Peptide Conjugates

被引：45
作者：
Collins, Jennifer [1 ]
Tanaka, Joji [1 ]
Wilson, Paul [1 ,2 ]
Kempe, Kristian [1 ]
Davis, Thomas P. [1 ,2 ]
McIntosh, Michelle P. [2 ]
Whittaker, Michael R. [2 ]
Haddleton, David M. [1 ,2 ]
机构：
[1] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England
[2] Monash Univ, Monash Inst Pharmaceut Sci, ARC Ctr Excellence Convergent Bionano Sci & Techn, Parkville, Vic 3052, Australia
来源：
BIOCONJUGATE CHEMISTRY | 2015年 / 26卷 / 04期
基金：
英国工程与自然科学研究理事会; 澳大利亚研究理事会;
关键词：
DIVALENT METAL-IONS; DRUG-DELIVERY; STABILIZE OXYTOCIN; AQUEOUS-SOLUTION; DISULFIDE BONDS; PEGYLATION; BIOCONJUGATION; PROTEINS; BUFFER; BROMOMALEIMIDES;
D O I：
10.1021/bc5006202
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The in situ one-pot synthesis of peptidepolymer bioconjugates is reported. Conjugation occurs efficiently without the need for purification of dithiophenol maleimide functionalized polymer as a disulfide bridging agent for the therapeutic oxytocin. Conjugation of polymers was demonstrated to be reversible and to significantly improve the solution stability of oxytocin.
引用
收藏
页码：633 / 638
页数：6
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