Mismatch repair detection (MRD): high-throughput scanning for DNA variations

被引：10

作者：

Faham, M ^{[1
]}

Baharloo, S ^{[1
]}

Tomitaka, S ^{[1
]}

DeYoung, J ^{[1
]}

Freimer, NB ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Psychiat, Neurogenet Lab, San Francisco, CA 94143 USA

来源：

HUMAN MOLECULAR GENETICS | 2001年 / 10卷 / 16期

关键词：

D O I：

10.1093/hmg/10.16.1657

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although there are several methods for genotyping previously identified single nucleotide polymorphisms (SNPs), there is a paucity of approaches for high-throughput scanning for unknown variations. Mismatch repair detection (MRD) utilizes a bacterial mismatch repair system in vivo to detect sequence variants in human DNA samples. We describe modifications in MRD that allow a high degree of parallel processing, and use this modified version to accurately scan for variations in 35 different human DNA fragments simultaneously. MRD's potential for high-throughput scanning can be used to identify new SNPs and to comprehensively compare sequences between patients and controls for identifying disease susceptibility alleles.

引用

页码：1657 / 1664

页数：8

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