Parylene-C coated micro-apertures with painted synthetic lipid bilayer membranes for the investigation of outer-membrane-vesicle fusion

被引：2

作者：

Ahmed, Tanzir ^{[1
]}

van den Driesche, Sander ^{[1
]}

Bafna, Jayesh Arun ^{[3
]}

Oellers, Martin ^{[1
]}

Hemmler, Roland ^{[2
]}

Gall, Karsten ^{[2
]}

Wagner, Richard ^{[3
]}

Winterhalter, Mathias ^{[3
]}

Vellekoop, Michael J. ^{[1
]}

机构：

[1] Univ Bremen, Inst Microsensors Actuators & Syst IMSAS, Microsyst Ctr Bremen MCB, Bremen, Germany

[2] Ionovation GmbH, D-49143 Bissendorf, Germany

[3] Jacobs Univ, Dept Life Sci & Chem, D-28759 Bremen, Germany

来源：

2019 IEEE SENSORS | 2019年

关键词：

Lipid bilayer; Parylene-C; Silicon; Silicon Nitride; OMV; CHANNELS;

D O I：

10.1109/sensors43011.2019.8956698

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We present a Parylene-C coated aperture realized in a silicon/silicon-nitride substrate to investigate the fusion of outer-membrane-vesicles (OMV) in synthetic lipid bilayers (LBL). The finalized chip was realized by employing standard silicon nitride membrane fabrication methods and the aperture was coated with 10 mu m Parylene-C. It makes the chip surface hydrophobic and lipophilic, preventing time consuming pretreatments (e.g. with organic solvents) before the formation of the LBL. Within a few seconds the LBL is formed. Our experiments show that the Parylene-C insulation is highly sufficient to achieve a low noise performance (1 pA RMS noise or ca. 2 to 4 pA peak-to-peak) at a 10 kHz sampling frequency and low-pass filtering at 1 kHz. Due to the quick LBL construction, the fusion of OMVs, extracted from the outer cellular wall of gram-negative bacteria took place within a few minutes. In addition, the proposed microfluidic system effectively detects the fast (1 ms) monomeric gating of outer membrane protein F (OmpF) pore proteins.

引用

页数：4