DNA barcoding of the fungal genus Neonectria and the discovery of two new species

被引：16

作者：

Zhao Peng ^{[1
,2
]}

Luo Jing ^{[1
]}

Zhuang WenYing ^{[1
]}

Liu XingZhong ^{[1
]}

Wu Bing ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

来源：

SCIENCE CHINA-LIFE SCIENCES | 2011年 / 54卷 / 07期

基金：

中国国家自然科学基金;

关键词：

intra-specific variation; inter-specific variation; morphology; DNA barcode; sequence analysis; PHYLOGENETIC-RELATIONSHIPS; PRIMER SETS; IDENTIFICATION; CYLINDROCARPON; GENES; HYPOCREALES; NECTRIACEAE; NUCLEAR; FOREST; RPB2;

D O I：

10.1007/s11427-011-4184-8

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To determine a suitable DNA barcode for the genus Neonectria, the internal transcribed spacer rDNA, beta-tubulin, EF-1 alpha, and RPB2 genes were selected as candidate markers. A total of 205 sequences from 19 species of the genus were analyzed. Intra- and inter-specific divergences and the ease of nucleotide sequence acquisition were treated as criteria to evaluate the feasibility of a DNA barcode. Our results indicated that any single gene among the candidate markers failed to serve as a successful barcode, while the combination of the partial EF-1 alpha, and RPB2 genes recognized all species tested. We tentatively propose the combined partial EF-1 alpha and RPB2 genes as a DNA barcode for the genus. During this study, two cryptic species were discovered, based on the combined data of morphology and DNA barcode information. We described and named these two new species N. ditissimopsis and N. microconidia.

引用

页码：664 / 674

页数：11

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