Performance-enhanced cholesterol biosensor based on biocomposite system: Layered double hydroxides-chitosan

被引：23

作者：

Ding, Shou-Nian ^{[1
,2
]}

Shan, Dan ^{[2
,3
]}

Zhang, Tao ^{[3
]}

Dou, Yan-Zhi ^{[3
]}

机构：

[1] Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Peoples R China

[2] Key Lab Environm Mat & Environm Engn Jiangsu Prov, Yangzhou 225002, Peoples R China

[3] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2011年 / 659卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Cholesterol oxidase; Horseradish peroxidase; Layered double hydroxides; Chitosan; Cholesterol; Biosensor; AMPEROMETRIC PHENOL BIOSENSOR; GLUCOSE-OXIDASE; COMPOSITE; NANOPARTICLES; MATRIX; LAPONITE; PLATFORM; DESIGN;

D O I：

10.1016/j.jelechem.2011.04.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Using layered double hydroxides (LDHs)-chitosan (Chit) composite as enzyme immobilization matrix, the developments of cholesterol biosensor were achieved by both electrooxidation and electroreduction of the enzymatic-generated hydrogen peroxide based on cholesterol oxidase (ChOx) and horseradish peroxidase (HRP). Amperometric detection of cholesterol was evaluated by holding the modified electrode at 0.55 V and 0 V (vs. SCE), for ChOx/LDHs-Chit/Pt and ChOx/HRP/LDHs-Chit/GCE, respectively. The linear dynamic ranges of anodic and cathodic detections of cholesterol were between 5 x 10(-7) to 8 x 10(-4) M and 4 x 10(-8) to 6 x 10(-4) M, respectively. The detection limits were determined to be of 1 x 10(-7) and 4 x 10(-8) M with anodic and cathodic processes, respectively. At potential of 0 V, ChOx/HRP/LDHs-Chit/GCE bienzymatic mediated system exhibited excellent selectivity and stability. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1 / 5

页数：5

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