Performance-enhanced cholesterol biosensor based on biocomposite system: Layered double hydroxides-chitosan

被引：23
作者：
Ding, Shou-Nian [1 ,2 ]
Shan, Dan [2 ,3 ]
Zhang, Tao [3 ]
Dou, Yan-Zhi [3 ]
机构：
[1] Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Peoples R China
[2] Key Lab Environm Mat & Environm Engn Jiangsu Prov, Yangzhou 225002, Peoples R China
[3] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China
来源：
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2011年 / 659卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Cholesterol oxidase; Horseradish peroxidase; Layered double hydroxides; Chitosan; Cholesterol; Biosensor; AMPEROMETRIC PHENOL BIOSENSOR; GLUCOSE-OXIDASE; COMPOSITE; NANOPARTICLES; MATRIX; LAPONITE; PLATFORM; DESIGN;
D O I：
10.1016/j.jelechem.2011.04.003
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Using layered double hydroxides (LDHs)-chitosan (Chit) composite as enzyme immobilization matrix, the developments of cholesterol biosensor were achieved by both electrooxidation and electroreduction of the enzymatic-generated hydrogen peroxide based on cholesterol oxidase (ChOx) and horseradish peroxidase (HRP). Amperometric detection of cholesterol was evaluated by holding the modified electrode at 0.55 V and 0 V (vs. SCE), for ChOx/LDHs-Chit/Pt and ChOx/HRP/LDHs-Chit/GCE, respectively. The linear dynamic ranges of anodic and cathodic detections of cholesterol were between 5 x 10(-7) to 8 x 10(-4) M and 4 x 10(-8) to 6 x 10(-4) M, respectively. The detection limits were determined to be of 1 x 10(-7) and 4 x 10(-8) M with anodic and cathodic processes, respectively. At potential of 0 V, ChOx/HRP/LDHs-Chit/GCE bienzymatic mediated system exhibited excellent selectivity and stability. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：1 / 5
页数：5
相关论文
共 26 条
[1]   Sensitive estimation of total cholesterol in blood using Au nanowires based micro-fluidic platform [J].
Aravamudhan, Shyam ;
Kumar, Arun ;
Mohapatra, Shyam ;
Bhansali, Shekhar .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (9-10) :2289-2294
[2]   Recent advances in cholesterol biosensor [J].
Arya, Sunil K. ;
Datta, Monika ;
Malhotra, Bansi D. .
BIOSENSORS & BIOELECTRONICS, 2008, 23 (07) :1083-1100
[3]   Specific determination of As(V) by an acid phosphatase-polyphenol oxidase biosensor [J].
Cosnier, Serge ;
Mousty, Christine ;
Cui, Xiaoqiang ;
Yang, Xiurong ;
Dong, Shaojun .
ANALYTICAL CHEMISTRY, 2006, 78 (14) :4985-4989
[4]  
de Roy A., 1992, ANIONIC CLAYS, P108, DOI [DOI 10.1007/978-1-4684-8866-1_7, 10.1007/978-1-4684-8866-1_7]
[5]   Preparation of polyaniline/multiwalled carbon nanotube composite by novel electrophoretic route [J].
Dhand, Chetna ;
Arya, Sunil K. ;
Singh, Surinder Pal ;
Singh, Bhanu Pratap ;
Datta, Monika ;
Malhotra, B. D. .
CARBON, 2008, 46 (13) :1727-1735
[6]   Glucose Oxidase Immobilized in Alginate/Layered Double Hydroxides Hybrid Membrane and Its Biosensing Application [J].
Ding, Shou-Nian ;
Shan, Dan ;
Xue, Huai-Guo ;
Zhu, Dao-Bin ;
Cosnier, Serge .
ANALYTICAL SCIENCES, 2009, 25 (12) :1421-1425
[7]   Amperometric phenol biosensor based on laponite clay-chitosan nanocomposite matrix [J].
Fan, Quan ;
Shan, Dan ;
Xue, Huaiguo ;
He, Yuanyuan ;
Cosnier, Serge .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (06) :816-821
[8]   Development of a stable cholesterol biosensor based on multi-walled carbon nanotubes-gold nanoparticles composite covered with a layer of chitosan-room-temperature ionic liquid network [J].
Gopalan, Anantha Iyengar ;
Lee, Kwang-Pill ;
Ragupathy, Dhanusuraman .
BIOSENSORS & BIOELECTRONICS, 2009, 24 (07) :2211-2217
[9]   Hybrid material based on chitosan and layered double hydroxides: Characterization and application to the design of amperometric phenol biosensor [J].
Han, En ;
Shan, Dan ;
Xue, Huaiguo ;
Cosnier, Serge .
BIOMACROMOLECULES, 2007, 8 (03) :971-975
[10]   Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection [J].
Kaushik, Ajeet ;
Solanki, Pratima R. ;
Kaneto, Keiichi ;
Kim, C. G. ;
Ahmad, Sharif ;
Malhotra, Bansi D. .
ELECTROANALYSIS, 2010, 22 (10) :1045-1055
123