In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell

被引：17

作者：

Prabu, P. ^{[2
]}

Chaudhari, Atul A. ^{[3
]}

Aryal, Santosh ^{[4
]}

Dharmaraj, N. ^{[5
]}

Park, S. Y. ^{[3
]}

Kim, W. D. ^{[6
]}

Kim, H. Y. ^{[1
]}

机构：

[1] Chonbuk Natl Univ, Dept Text Engn, Jeonju 561756, South Korea

[2] Chonbuk Natl Univ, Dept Bionanosyst Engn, Jeonju 561756, South Korea

[3] Chonbuk Natl Univ, Coll Vet Med, Jeonju 561756, South Korea

[4] Chonbuk Natl Univ, Ctr Healthcare Technol Dev, Jeonju 561756, South Korea

[5] Bharathiar Univ, Dept Chem, Coimbatore 641046, Tamil Nadu, India

[6] Korea Inst Machinery & Mat, Dept Future Technol, Taejon 305343, South Korea

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2008年 / 19卷 / 05期

关键词：

D O I：

10.1007/s10856-007-3307-z

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This study dealt with the preparation and characterization of coumarin-6 loaded poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of cellular uptake by using human gastric cancer cell line (SNU-638), in vitro. The potential application of these PGD NPs for sustained drug delivery was evaluated by the quantification and localization of the cellular uptake of fluorescent PGD NPs. Coumarin-6 loaded PGD NPs were prepared by a modified oil/water emulsion technique and characterized by various physico-chemical methods such as, laser light scattering for particle size and size distribution, atomic force microscopy (AFM), zeta-potential and spectrofluorometry to identify the release of fluorescent molecules from the NPs. SNU-638 was used to measure the cellular uptake of fluorescent PGD NPs. Confocal laser scanning microscopic images clearly showed the internalization of NPs by the SNU-638 cells. Cell viability was assessed by treating the SNU-638 cells with PGD NPs for 48 h. The results reveal, that these biodegradable polymeric NPs holds promise in biomedical field as a carrier.

引用

页码：2157 / 2163

页数：7

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