Protein adsorption on hydroxyapatite nano-crystals with quartz crystal microbalance technique

被引：17

作者：

Yoshioka, T. ^{[1
]}

Ikoma, T. ^{[2
]}

Monkawa, A. ^{[2
]}

Tonegawa, T. ^{[1
]}

Chakarov, D. ^{[3
]}

Kasemo, B. ^{[3
]}

Hanagata, N. ^{[2
]}

Tanaka, J. ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Met & Ceram Engn, Meguro Ku, 2-12-1 Ookayama, Tokyo 1528550, Japan

[2] Natl Inst Mat Sci, Biomat Sci, Tsukuba, Ibaraki 3050044, Japan

[3] Chalmers Univ Technol, Dept Appl Phys, S-41296 Gothenburg, Sweden

来源：

BIOCERAMICS, VOL 20, PTS 1 AND 2 | 2008年 / 361-363卷

关键词：

hydroxyapatite (HAp); nanocrystal; protein adsorption; QCM-D;

D O I：

10.4028/www.scientific.net/KEM.361-363.1119

中图分类号：

TQ174 [陶瓷工业]; TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Real time adsorption behaviors of six proteins with different isoelectric points on hydroxyapatite (HAp) nanocrystal surfaces have been investigated by using HAp sensors for quartz crystal microbalance with dissipation technique (QCM-D). The dissipation (D)-frequency (f) plots clearly showed that the different types of protein adsorption behaviors; the D-f plots of acidic proteins lie on one straight line with a constant slope under all initial protein concentrations, while those of neutral and basic proteins lie on two straight lines with different slopes. The acidic proteins formed a monolayer, while the neutral and basic proteins could cause conformational changes with the adsorbed amount of proteins. The QCM-D technique with novel HAp nanocrystal sensor is useful for the liquid phase changes of proteins on the surface.

引用

页码：1119 / +

页数：2

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