Human Papillomavirus E6/E7 Expression in Preeclampsia-Affected Placentae

被引:5
作者
Reily-Bell, Ashley L. [1 ]
Fisher, Amanda [1 ]
Harrison, Bryony [1 ]
Bowie, Sara [1 ]
Ray, Sankalita [1 ]
Hawkes, Mary [1 ]
Wise, Lyn M. [2 ]
Fukuzawa, Ryuji [1 ,3 ]
Macaulay, Erin C. [1 ]
Devenish, Celia J. [4 ]
Hung, Noelyn A. [1 ]
Slatter, Tania L. [1 ,5 ]
机构
[1] Univ Otago, Dunedin Sch Med, Dept Pathol, Dunedin 9016, New Zealand
[2] Univ Otago, Sch Biomed Sci, Dept Pharmacol & Toxicol, Dunedin 9016, New Zealand
[3] Int Univ Hlth & Welf, Sch Med, Dept Pathol, Narita 2868686, Japan
[4] Univ Otago, Dunedin Sch Med, Dept Womens & Childrens Hlth, Dunedin 9016, New Zealand
[5] Maurice Wilkins Ctr Mol Biodiscovery, Auckland 1142, New Zealand
关键词
HPV; E6; E7; preeclampsia; placenta; trophoblast; RNAscope; SPONTANEOUSLY ABORTED PRODUCTS; CARDIOVASCULAR-DISEASE; MESSENGER-RNA; LIFE-CYCLE; HIGH-RISK; INFECTION; HPV; E6; WOMEN; PREGNANCY;
D O I
10.3390/pathogens9030239
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Whether HPV is causative of pregnancy complications is uncertain. E6 and E7 affect functions underling preeclampsia (PET) in cultured trophoblasts, but whether E6 and E7 is produced in the placenta is uncertain. Here, we investigated whether E6/E7 was expressed in the placentae from pregnancies with PET, other pregnancy complications (fetal growth restriction (FGR) and diabetes mellitus), and uncomplicated pregnancies. Placental tissues collected from two geographical locations were subjected to RNAscope analyses of high- and low- risk E6/E7, and individual HPV types identified using an HPV array. High-risk E6/E7 expression was increased in both PET cohorts, (81% and 86% of patients positive for high-risk HPV DNA compared to 13% of control patients). Various HPV types were identified. Although HPV 18 was the most frequent in all cohorts, the majority of individuals had multiple HPV types (55% of the PET compared to 25% of the control cohort). Further evidence that E6 and E7 is present early when placental pathology underlying preeclampsia is established, is provided with the finding of high-risk E6/E7 in the first-trimester placenta anchoring trophoblast. In conclusion, E6/E7 expression and multiple HPV types were frequent in placentae from preeclampsia-complicated pregnancies.
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页数:18
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