Ferritin as a photocatalyst and scaffold for gold nanoparticle synthesis

被引:32
作者
Keyes, Jeremiah D. [1 ]
Hilton, Robert J. [1 ]
Farrer, Jeffrey [2 ]
Watt, Richard K. [1 ]
机构
[1] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA
[2] Brigham Young Univ, Dept Phys & Astron, Provo, UT 84602 USA
关键词
Gold nanoparticles; Ferritin; Nanoparticle synthesis; Photochemistry; Photoreduction; HORSE SPLEEN; PROTEIN CAGE; APOFERRITIN CAVITY; MAMMALIAN FERRITIN; REDOX REACTIONS; BIO-TEMPLATE; FABRICATION; OXIDE; DNA; NANOREACTOR;
D O I
10.1007/s11051-010-0149-2
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The ferrihydrite mineral core of ferritin is a semi-conductor capable of catalyzing oxidation/reduction reactions. This report shows that ferritin can photoreduce AuCl4 (-) to form gold nanoparticles (AuNPs). An important goal was to identify innocent reaction conditions that prevented formation of AuNPs unless the sample was illuminated in the presence of ferritin. TRIS buffer satisfied this requirement and produced AuNPs with spherical morphology with diameters of 5.7 +/- A 1.6 nm and a surface plasmon resonance (SPR) peak at 530 nm. Size-exclusion chromatography of the AuNP-ferritin reaction mixture produced two fractions containing both ferritin and AuNPs. TEM analysis of the fraction close to where native ferritin normally elutes showed that AuNPs form inside ferritin. The other peak eluted at a volume indicating a particle size much larger than ferritin. TEM analysis revealed AuNPs adjacent to ferritin molecules suggesting that a dimeric ferritin-AuNP species forms. We propose that the ferritin protein shell acts as a nucleation site for AuNP formation leading to the AuNP-ferritin dimeric species. Ferrihydrite nanoparticles (similar to 10 nm diameter) were unable to produce soluble AuNPs under identical conditions unless apo ferritin was present indicating that the ferritin protein shell was essential for stabilizing AuNPs in aqueous solution.
引用
收藏
页码:2563 / 2575
页数:13
相关论文
共 64 条
  • [1] Ferritins: A family of molecules for iron storage, antioxidation and more
    Arosio, Paolo
    Ingrassia, Rosaria
    Cavadini, Patrizia
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2009, 1790 (07): : 589 - 599
  • [2] CVD synthesis of single-walled carbon nanotubes from gold nanoparticle catalysts
    Bhaviripudi, Sreekar
    Mile, Ervin
    Steiner, Stephen A., III
    Zare, Aurea T.
    Dresselhaus, Mildred S.
    Belcher, Angela M.
    Kong, Jing
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (06) : 1516 - +
  • [3] Directing Noble Metal Ion Chemistry within a Designed Ferritin Protein
    Butts, Christopher A.
    Swift, Joe
    Kang, Seung-gu
    Di Costanzo, Luigi
    Christianson, David W.
    Saven, Jeffery G.
    Dmochowski, Ivan J.
    [J]. BIOCHEMISTRY, 2008, 47 (48) : 12729 - 12739
  • [4] Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection
    Cao, YWC
    Jin, RC
    Mirkin, CA
    [J]. SCIENCE, 2002, 297 (5586) : 1536 - 1540
  • [5] Direct electron transfer of ferritin adsorbed at tin-doped indium oxide electrodes
    Cherry, RJ
    Bjornsen, AJ
    Zapien, DC
    [J]. LANGMUIR, 1998, 14 (08) : 1971 - 1973
  • [6] Amyloid-β detection with saccharide immobilized gold nanoparticle on carbon electrode
    Chikae, Miyuki
    Fukuda, Tomohiro
    Kerman, Kagan
    Idegami, Koutarou
    Miura, Yoshiko
    Tamiya, Eiichi
    [J]. BIOELECTROCHEMISTRY, 2008, 74 (01) : 118 - 123
  • [7] Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology
    Daniel, MC
    Astruc, D
    [J]. CHEMICAL REVIEWS, 2004, 104 (01) : 293 - 346
  • [8] Size-controlled water-soluble Ag nanoparticles
    Dominguez-Vera, Jose M.
    Galvez, Natividad
    Sanchez, Purificacion
    Mota, Antonio J.
    Trasobares, Susana
    Hernandez, Juan C.
    Calvino, Jose J.
    [J]. EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2007, 30 (30) : 4823 - 4826
  • [9] SYNTHESIS AND STRUCTURE OF AN IRON(III) SULFIDE-FERRITIN BIOINORGANIC NANOCOMPOSITE
    DOUGLAS, T
    DICKSON, DPE
    BETTERIDGE, S
    CHARNOCK, J
    GARNER, CD
    MANN, S
    [J]. SCIENCE, 1995, 269 (5220) : 54 - 57
  • [10] Nanophase cobalt oxyhydroxide mineral synthesized within the protein cage of ferritin
    Douglas, T
    Stark, VT
    [J]. INORGANIC CHEMISTRY, 2000, 39 (08) : 1828 - 1830