Irisflorentin Modifies Properties of Mouse Bone Marrow-Derived Dendritic Cells and Reduces the Allergic Contact Hypersensitivity Responses

被引：16

作者：

Fu, Ru-Huei ^{[1
,2
,3
]}

Tsai, Chia-Wen ^{[4
]}

Tsai, Rong-Tzong ^{[5
]}

Liu, Shih-Ping ^{[2
,6
]}

Chan, Tzu-Min ^{[7
,8
]}

Ho, Yu-Chen ^{[1
]}

Lin, Hsin-Lien ^{[1
]}

Chen, Yue-Mi ^{[1
]}

Hung, Huey-Shan ^{[2
,6
]}

Chiu, Shao-Chih ^{[1
,2
]}

Tsai, Chang-Hai ^{[9
]}

Wang, Yu-Chi ^{[10
]}

Shyu, Woei-Cherng ^{[1
,2
]}

Lin, Shinn-Zong ^{[1
,2
,7
,8
]}

机构：

[1] China Med Univ, Grad Inst Immunol, Taichung 40402, Taiwan

[2] China Med Univ Hosp, Ctr Neuropsychiat, Taichung, Taiwan

[3] Asia Univ, Dept Psychol, Taichung, Taiwan

[4] China Med Univ, Dept Nutr, Taichung, Taiwan

[5] Chung Shan Med Univ, Inst Biochem & Biotechnol, Taichung, Taiwan

[6] China Med Univ, Grad Inst Basic Med Sci, Taichung, Taiwan

[7] China Med Univ, Beigang Hosp, Dept Neurosurg, Yunlin, Taiwan

[8] China Med Univ, Tainan Municipal An Nan Hosp, Dept Neurosurg, Tainan, Taiwan

[9] China Med Univ, Dept Pediat, Taichung, Taiwan

[10] Ind Technol Res Inst, Biomed Technol & Device Res Labs, Hsinchu, Taiwan

来源：

CELL TRANSPLANTATION | 2015年 / 24卷 / 03期

关键词：

Dendritic cells (DCs); Immunosuppression; Irisflorentin; Nuclear factor-kappa B (NF-kappa B); Mitogen-activated protein kinase (MAPK); Contact hypersensitivity response (CHSR); NF-KAPPA-B; REGULATORY T-CELLS; BELAMCANDA-CHINENSIS; IMMUNOSTIMULATORY FUNCTION; SKIN; ISOFLAVONES; MACROPHAGES; MODULATION; DERMATITIS; DC;

D O I：

10.3727/096368915X687002

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Irisflorentin is an isoflavone component derived from the roots of Belamcanda chinensis (L.) DC. In traditional Chinese medicine, this herb has pharmacological properties to treat inflammatory disorders. Dendritic cells (DCs) are crucial modulators for the development of optimal T-cell immunity and maintenance of tolerance. Aberrant activation of DCs can induce harmful immune responses, and so agents that effectively improve DC properties have great clinical value. We herein investigated the effects of irisflorentin on lipopolysaccharide (LPS)-stimulated maturation of mouse bone marrow-derived DCs in vitro and in the contact hypersensitivity response (CHSR) in vivo. Our results demonstrated that treatment with up to 40 mu M irisflorentin does not cause cellular toxicity. Irisflorentin significantly lessened the proinflammatory cytokine production (tumor necrosis factor-alpha, interleuldn-6, and interleuldn-12p70) by LPS-stimulated DCs. Irisflorentin also inhibited the expression of LPS-induced major histocompatibility complex class II and costimulatory molecules (CD40 and CD 86) on LPS-stimulated DCs. In addition, irisflorentin diminished LPS-stimulated DC-elicited allogeneic T-cell proliferation. Furthermore, irisflorentin significantly interfered with LPS-induced activation of I kappa B kinase, c-Jun N-terminal kinase, and p38, as well as the nuclear translocation of NF-kappa B p65. Subsequently, treatment with irisflorentin obviously weakened 2,4-dinitro-1-fluorobenzene-induced delayed-type hypersensitivity. These findings suggest new insights into the role of irisflorentin as an immunotherapeutic adjuvant through its capability to modulate the properties of DCs.

引用

页码：573 / 588

页数：16

共 39 条

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