Microchip electrospray: Cone-jet stability analysis for water-acetonitrile and water-methanol mobile phases

被引：5

作者：

Jung, Stephanie ^{[1
]}

Effelsberg, Uwe ^{[2
]}

Tallarek, Ulrich ^{[1
]}

机构：

[1] Univ Marburg, Dept Chem, D-35032 Marburg, Germany

[2] Agilent Technol, D-76337 Waldbronn, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2011年 / 1218卷 / 12期

关键词：

Microchip HPLC/ESI-MS; Electrospray modes; Cone-jet stability island; Gradient elution; Bulk conductivity; Organic modifier content; Flow rate effect; MASS-SPECTROMETRY; NANOFLOW REGIME; PERFORMANCE; IONIZATION; MIXTURES; MODES; CHIP; FLOW; MICROFLUIDICS; SEPARATIONS;

D O I：

10.1016/j.chroma.2011.01.054

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in mobile phase composition during high performance liquid chromatography (HPLC) gradient elution coupled to mass spectrometry (MS) sensitively affect electrospray operation modes. In this work, we identify the influences of dynamic changes in bulk conductivity on the cone-jet stability island for aqueous acetonitrile and aqueous methanol mobile phases commonly used in reversed-phase HPLC. Bulk conductivities of the mobile phases were varied by adding different amounts of formic acid. A commercial microchip-HPLC/ESI-MS configuration was modified to enable in situ electrospray diagnostics by frequency analysis of the microchip emitter current and spray imaging. This approach facilitated the detection of different spray modes together with their onset potentials. The established spray modes are described and the differences in onset potentials and stability regions explained by the physicochemical properties of the electrosprayed liquid. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1611 / 1619

页数：9

共 49 条

[1] Dielectric constants of some organic solvent-water mixtures at various temperatures
Akerlof, G
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1932, 54 : 4125 - 4139
[2] [Anonymous], 1961, J CHEM ENG DATA, DOI [DOI 10.1021/JE60011A026, DOI 10.1021/JE60011A015]
[3] Latest Developments in Micro Total Analysis Systems
Arora, Arun
Simone, Giuseppina
Salieb-Beugelaar, Georgette B.
Kim, Jung Tae
Manz, Andreas
[J]. ANALYTICAL CHEMISTRY, 2010, 82 (12) : 4830 - 4847
[4] Microfluidic devices for sample pretreatment and applications
Chen, Xing
Cui, Da-Fu
[J]. MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS, 2009, 15 (05): : 667 - 676
[5] ELECTROSTATIC SPRAYING OF LIQUIDS IN CONE-JET MODE
CLOUPEAU, M
PRUNETFOCH, B
[J]. JOURNAL OF ELECTROSTATICS, 1989, 22 (02) : 135 - 159
[6] ELECTROHYDRODYNAMIC SPRAYING FUNCTIONING MODES - A CRITICAL-REVIEW
CLOUPEAU, M
PRUNETFOCH, B
[J]. JOURNAL OF AEROSOL SCIENCE, 1994, 25 (06) : 1021 - 1036
[7] ROLE OF THE TEMPERATURE IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING PYROCARBON-CONTAINING ADSORBENTS
COLIN, H
DIEZMASA, JC
GUIOCHON, G
CZAJKOWSKA, T
MIEDZIAK, I
[J]. JOURNAL OF CHROMATOGRAPHY, 1978, 167 (DEC): : 41 - 65
[8] Charge and size distributions of electrospray drops
deJuan, L
delaMora, JF
[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1997, 186 (02) : 280 - 293
[9] THE CURRENT EMITTED BY HIGHLY CONDUCTING TAYLOR CONES
DELAMORA, JF
LOSCERTALES, IG
[J]. JOURNAL OF FLUID MECHANICS, 1994, 260 : 155 - 184
[10] Multiplexed detection and applications for separations on parallel microchips
Dishinger, John F.
Kennedy, Robert T.
[J]. ELECTROPHORESIS, 2008, 29 (16) : 3296 - 3305

← 1 2 3 4 5 →