Macrophage responses to selective laser-melted Ti-6Al-4V scaffolds of different pore geometries and the corresponding osteoimmunomodulatory effects toward osteogenesis

被引:10
|
作者
Wu, Yun-Qi [1 ,2 ,3 ]
Liu, Zheng [4 ]
Xu, Zhenchao [1 ]
Zhang, Yilu [1 ]
Ye, Hongru [1 ]
Wang, Xiyang [1 ,2 ,3 ,5 ]
机构
[1] Cent South Univ, Xiangya Hosp, Hunan Engn Lab Adv Artificial Osteomat, 87 Xiangya Rd, Changsha 410008, Hunan, Peoples R China
[2] Cent South Univ, Xiangya Hosp, Dept Spine Surg & Orthopaed, Changsha, Peoples R China
[3] Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Peoples R China
[4] Hunan Childrens Hosp, Dept Orthoped Surg, Changsha, Peoples R China
[5] Natl Univ Def Technol, Sci & Technol Adv Ceram Fibers & Composites Lab, Changsha, Peoples R China
关键词
macrophages; orthopedic implants; osteogenesis; osteoimmunology; scaffolds; POROUS TI6AL4V SCAFFOLD; ALKALI-ACID-HEAT; IN-VITRO; MECHANICAL-PROPERTIES; POLARIZATION; DESIGN; SECRETION; IMPLANTS; IMPACT; CELLS;
D O I
10.1002/jbm.a.37335
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Following recent advances in osteoimmunology, there is growing recognition of the vital role of immune cells in the osteogenesis process. The 3D-printed scaffold, as a substitute for injured and/or diseased bone tissues, has demonstrated satisfactory pro-osteogenetic performance. However, whether immune cells prompt the above pro-osteogenetic performance has not been elucidated in detail. In the present study, highly controllable Ti-6Al-4V scaffolds with different pore geometries were fabricated using a selective laser-melting technique, to reveal their osteoimmunological functions with macrophages. The results showed that macrophages displayed characteristics of M2 phenotype in response to scaffolds. As a result, an anti-inflammatory microenvironment was generated. When the pore geometry was considered, such observations were more apparent with the hexagonal pore scaffold than with the triangular one. In addition, inhibition of the toll-like receptor signaling pathway in macrophages has been proposed to cause the above phenomenon. Upon applying conditioned media derived from macrophages on pre-osteoblasts, the hexagonal pore scaffold group was found to significantly enhance osteoblastic differentiation, via macrophage-to-implant interactions. However, the effect of triangular pore scaffold was not statistically significant compared to that of hexagonal pore scaffolds or nonporous samples. In an attempt to quantify scaffold pore geometries, it was suggested that pores with higher circularity values tended to induce M2 polarization of macrophages, promote an anti-inflammatory milieu, and therefore, achieve better osteogenetic performance via immunomodulation.
引用
收藏
页码:873 / 883
页数:11
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