Facile green synthesis of non-genotoxic, non-hemolytic organometallic silver nanoparticles using extract of crushed, wasted, and spent Humulus lupulus (hops): Characterization, anti-bacterial, and anti-cancer studies

被引:50
作者
Das, Pratik [1 ]
Dutta, Tanusree [1 ]
Manna, Suvendu [1 ,2 ]
Loganathan, Sravanthi [3 ]
Basak, Piyali [1 ]
机构
[1] Jadavpur Univ, Sch Biosci & Engn, Kolkata 700032, W Bengal, India
[2] Univ Petr & Energy Studies, Sch Engn, Dept Hlth Safety Environm & Civil Engn, Dehra Dun 247008, Uttarakhand, India
[3] CSIR Cent Electrochem Res Inst CECRI, Karaikkudi 630006, Tamil Nadu, India
关键词
Organometallic silver nanoparticles; Genotoxicity; Biological activity; Spent hops; Biocompatible silver nanoparticles; IN-VITRO; ANTIMICROBIAL ACTIVITY; BIOLOGICAL SYNTHESIS; POMEGRANATE EXTRACT; GOLD; CYTOTOXICITY; ANTIOXIDANT; TOXICITY; IDENTIFICATION; APOPTOSIS;
D O I
10.1016/j.envres.2021.111962
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Since the last few decades, the green synthesis of metal nanoparticles was one of the most thrust areas due to its widespread application. The study proposed using wasted and unusable Humulus lupulus (Hops) extract to synthesize silver nanoparticles for biomedical application. The environment around us gives us many scopes to use the waste from environmental sources and turn it into something valuable. The spent Hops extract was used to synthesize silver nanoparticles (AgNP@HOPs), and the synthesized product exhibited an excellent therapeutic effect in terms of anti-bacterial and anti-cancer agents. The synthesis was optimized considering different factors like time and the concentration of AgNO3. The silver nanoparticles were characterized in detail using different characterization techniques XRD, DLS, TEM, BET, XPS, Raman Spectroscopy, SEM, EDAX, AFM, which revealed the uniqueness of the silver nanoparticles. The average hydrodynamic size was found to be 92.42 +/- 2.41 with a low polydispersity index. The presence of Ag-C and Ag-O bonds in the AgNP@HOPs indicated that it is composed of organo-silver and silver oxides. The nanoparticles were found to be spherical with an average size of 17.40 nm. The AgNPs were lethal to both E. coli and S. aureus with a MIC-50 of 201.881 mu g/mL and 213.189 mu g/mL, respectively. The AgN-P@HOPs also exhibited an anti-cancer effect with an IC-50 of 147.175. The AgN-P@HOPs exhibited less cytotoxicity and genotoxicity against normal cells and exhibited superior haemo-compatibility (major criteria for drug selection). There are indeed various reports on the synthesis of silver nanoparticles, but this study proposes a green method for producing non-genotoxic, non-hemolytic organome-tallic silver nanoparticles using waste material with considerable therapeutic index from the environmental source with potential application in the medical industry. This work could be taken forward for in-vivo studies and for pre clinical studies.
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页数:19
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