Expression of the human -defensin-1 induced by dehydroandrographolide in human pulmonary gland epithelial cells

被引：3

作者：

Shao, Zhen-Jun ^{[1
]}

Li, Xing-Ping ^{[2
]}

Wu, Yi-Ying ^{[1
]}

Zheng, Xiao-Wei ^{[1
]}

Huang, Juan ^{[3
,4
]}

Deng, Wen-Long ^{[2
]}

Zhou, Li-Ming ^{[1
]}

机构：

[1] Sichuan Univ, Dept Pharmacol, W China Med Ctr, Preclin & Forens Med Coll, Chengdu 610041, Peoples R China

[2] Sichuan Acad Chinese Med Sci, Chengdu 610041, Peoples R China

[3] Sichuan Univ, Joint Res Ctr, W China Univ Hosp 2, Chengdu 610041, Peoples R China

[4] Univ Hong Kong, Fac Med, Chengdu 610041, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2010年 / 21卷 / 04期

关键词：

dehydroandrographolide; human; -defensin-1; human pulmonary gland epithelial cells; ANDROGRAPHIS-PANICULATA; BETA-DEFENSIN-2; DEFENSINS;

D O I：

10.1080/09540105.2010.514896

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study we observed the enhanced mRNA and protein expression of human -defensin-1 (hBD-1) induced by dehydroandrographolide (DA) in human gland epithelial cells and explored the mechanism of DA on infection treatment. Human pulmonary gland epithelial cells were incubated with DA, then were harvested for hBD-1 expression detection. The mRNA expression of hBD-1 was detected by RT-PCR while the protein expression was detected by Western blot. It was found that DA can up-regulate mRNA and protein expression of hBD-1, the optimal concentration was 80 mu M, the maximal expression of hBD-1 mRNA and protein occurred after 8 h. The DA can up-regulate mRNA and protein expression of hBD-1 in human gland epithelial cells. It suggests that -defensin-1 may play an important role in the treatment of infective diseases with DA.

引用

页码：361 / 367

页数：7

共 50 条

[41] Human alpha-defensin-1 protects cells from intoxication with Clostridium perfringens iota toxin
Fischer, Stephan
Popoff, Michel R.
Barth, Holger
PATHOGENS AND DISEASE, 2018, 76 (02):
[42] Proteomic analysis of human tears: Defensin expression after ocular surface surgery
Zhou, L
Huang, LQ
Beuerman, RW
Grigg, ME
Li, SFY
Chew, FT
Ang, L
Stern, ME
Tan, D
JOURNAL OF PROTEOME RESEARCH, 2004, 3 (03) : 410 - 416
[43] Effects of potential probiotics on the expression of cytokines and human β-defensins in human gingival epithelial cells and in vivo efficacy in a dog model
Pahumunto, Nuntiya
Duangnumsawang, Yada
Teanpaisan, Rawee
ARCHIVES OF ORAL BIOLOGY, 2022, 142
[44] Xenobiotic transporter expression and function in the human mammary gland
Ito, S
Alcorn, J
ADVANCED DRUG DELIVERY REVIEWS, 2003, 55 (05) : 653 - 665
[45] Variability of miRNA expression during the differentiation of human embryonic stem cells into retinal pigment epithelial cells
Yuan, Zhidong
Ding, Suping
Yan, Mingli
Zhu, Xiao
Liu, Lili
Tan, Shuhua
Jin, Yuanchang
Sun, Yuandong
Li, Yufeng
Huang, Ting
GENE, 2015, 569 (02) : 239 - 249
[46] Differential expression of the Quox-1 gene in normal human cells, early human embryo, and tumor cells
Zhu, F
Li, WX
Jiang, DH
Gou, DM
CELL AND TISSUE RESEARCH, 2002, 308 (02) : 333 - 337
[47] Effects of Human Defensin-α1 on Trypanosoma cruzi Trypomastigotes in Vitro
Kleschenko, Yu. E.
Karpenko, L. P.
Villalta, F.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2010, 149 (06) : 731 - 733
[48] β-defensin 1 gene variability among non-human primates
Del Pero, M
Boniotto, M
Zuccon, D
Cervella, P
Spanò, A
Amoroso, A
Crovella, S
IMMUNOGENETICS, 2002, 53 (10-11) : 907 - 913
[49] β-Defensin-3 and -4 in intestinal epithelial cells display increased mRNA expression in ulcerative colitis
Fahlgren, A
Hammarström, S
Danielsson, Å
Hammarström, ML
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2004, 137 (02) : 379 - 385
[50] Production of Bioactive Human β-defensin-3 in Escherichia coli by Soluble Fusion Expression
Lei Huang
Jufang Wang
Zhixia Zhong
Li Peng
Haiqin Chen
Zhinan Xu
Peilin Cen
Biotechnology Letters, 2006, 28 : 627 - 632

← 1 2 3 4 5 →