Expression of the human -defensin-1 induced by dehydroandrographolide in human pulmonary gland epithelial cells

被引：3

作者：

Shao, Zhen-Jun ^{[1
]}

Li, Xing-Ping ^{[2
]}

Wu, Yi-Ying ^{[1
]}

Zheng, Xiao-Wei ^{[1
]}

Huang, Juan ^{[3
,4
]}

Deng, Wen-Long ^{[2
]}

Zhou, Li-Ming ^{[1
]}

机构：

[1] Sichuan Univ, Dept Pharmacol, W China Med Ctr, Preclin & Forens Med Coll, Chengdu 610041, Peoples R China

[2] Sichuan Acad Chinese Med Sci, Chengdu 610041, Peoples R China

[3] Sichuan Univ, Joint Res Ctr, W China Univ Hosp 2, Chengdu 610041, Peoples R China

[4] Univ Hong Kong, Fac Med, Chengdu 610041, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2010年 / 21卷 / 04期

关键词：

dehydroandrographolide; human; -defensin-1; human pulmonary gland epithelial cells; ANDROGRAPHIS-PANICULATA; BETA-DEFENSIN-2; DEFENSINS;

D O I：

10.1080/09540105.2010.514896

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study we observed the enhanced mRNA and protein expression of human -defensin-1 (hBD-1) induced by dehydroandrographolide (DA) in human gland epithelial cells and explored the mechanism of DA on infection treatment. Human pulmonary gland epithelial cells were incubated with DA, then were harvested for hBD-1 expression detection. The mRNA expression of hBD-1 was detected by RT-PCR while the protein expression was detected by Western blot. It was found that DA can up-regulate mRNA and protein expression of hBD-1, the optimal concentration was 80 mu M, the maximal expression of hBD-1 mRNA and protein occurred after 8 h. The DA can up-regulate mRNA and protein expression of hBD-1 in human gland epithelial cells. It suggests that -defensin-1 may play an important role in the treatment of infective diseases with DA.

引用

页码：361 / 367

页数：7

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