Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis

被引：5
作者：
Hu, Tao [2 ]
Niu, Yunfeng [3 ]
Fu, Jianfeng [2 ]
Dong, Zhiming [3 ]
He, Dongwei [3 ]
Liu, Junfeng [1 ]
机构：
[1] Hebei Med Univ, Dept Thorac Surg, Hosp 4, Shijiazhuang, Hebei, Peoples R China
[2] Hebei Med Univ, Dept Anesthesiol, Hosp 4, Shijiazhuang, Hebei, Peoples R China
[3] Hebei Med Univ, Hebei Canc Inst, Lab Pathol, Hosp 4, Shijiazhuang, Hebei, Peoples R China
来源：
OPEN MEDICINE | 2022年 / 17卷 / 01期
关键词：
PCNA-AS1; PCNA; miR-2467-3p; ESCC; HIGH EXPRESSION; CANCER; PROLIFERATION; INVASION; MIGRATION; GROWTH;
D O I：
10.1515/med-2022-0552
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Multiple studies have indicated that long non-coding RNAs are aberrantly expressed in cancers and are pivotal in developing various tumors. No studies have investigated the expression and function of long non-coding antisense RNA PCNA-AS1 in esophageal squamous cell carcinoma (ESCC). In this study, the expression of PCNA-AS1 was identified by qRT-PCR. Cell function assays were used to explore the potential effect of PCNA-AS1 on ESCC progression. A prediction website was utilized to discover the relationships among PCNA-AS1, miR-2467-3p and proliferating cell nuclear antigen (PCNA). Dual luciferase reporter gene and RNA immunoprecipitation (RIP) assays were executed to verify the binding activity between PCNA-AS1, miR-2467-3p and PCNA. As a result, PCNA-AS1 was highly expressed in ESCC and was associated with patient prognosis. PCNA-AS1 overexpression strongly contributed to ESCC cell proliferation, invasion and migration. PCNA-AS1 and PCNA were positively correlated in ESCC. Bioinformatics analysis, RIP and luciferase reporter gene assays revealed that PCNA-AS1 could act as a competitive endogenous RNA to sponge miR-2467-3p, thus upregulating PCNA. In conclusion, the current outcome demonstrates that PCNA-AS1 may be a star molecule in the treatment of ESCC.
引用
收藏
页码：1483 / 1494
页数：12
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