Cloning and expression analysis of a cDNA coding for a dexamethasone-inducible cytochrome P450 in Xenopus laevis liver

被引：0

作者：

Ohi, H ^{[1
]}

Sugata, E ^{[1
]}

Fujita, Y ^{[1
]}

Saito, H ^{[1
]}

Saguchi, K ^{[1
]}

Murayama, N ^{[1
]}

Higuchi, S ^{[1
]}

机构：

[1] Showa Univ, Sch Pharmaceut Sci, Dept Biopharmaceut, Shinagawa Ku, Tokyo 142, Japan

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1998年 / 45卷 / 04期

关键词：

cytochrome P450; CYP2Q subfamily; dexamethasone; induction; degenerate PCR primers; 5 ' RACE; Xenopus laevis;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously purified a cytochrome P450 (P450) from liver microsomes of adult female Xenopus laevis. In this study, we screened a cDNA library of Xenopus liver to isolate the cDNA clone coding for this P450. The 5'-end of the resultant cDNA was truncated at the N-terminal region and extended by method of rapid amplification of cDNA end to give the complete coding sequence. Amino acid sequence showed this clone to be 36% to 55% identical to members of the CYP2 family and less than 31% identical to members of other gene families, and to belong to the CYP2Q subfamily. This gene is expressed constitutively in the livers of adult male and female frogs, and is significantly induced by dexamethasone administration.

引用

页码：689 / 697

页数：9

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