Cyclic AMP directly activates NasP, an N-acyl amino acid antibiotic biosynthetic enzyme cloned from an uncultured β-proteobacterium

被引：14

作者：

Clardy, Jon

Brady, Sean F.

机构：

[1] Rockefeller Univ, Lab Genet Encoded Small Mol, New York, NY 10021 USA

[2] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

JOURNAL OF BACTERIOLOGY | 2007年 / 189卷 / 17期

关键词：

D O I：

10.1128/JB.00457-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The cyclic AMP (cAMP)-dependent biosynthesis of N-acylphenylalanine antibiotics by NasP, an environmental DNA-derived N-acyl amino acid synthase, is controlled by an NasP-associated cyclic nucleotide-binding domain and is independent of the global cAMP signal transducer, cAMP receptor protein. A 16S rRNA gene sequence found on the same environmental DNA cosmid as NasP is most closely related to 16S sequences from beta-proteobacteria.

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页码：6487 / 6489

页数：3

相关论文

共 26 条

[1] Environmental whole-genome amplification to access microbial populations in contaminated sediments [J].

Abulencia, Carl B. ;

Wyborski, Denise L. ;

Garcia, Joseph A. ;

Podar, Mircea ;

Chen, Wenqiong ;

Chang, Sherman H. ;

Chang, Hwai W. ;

Watson, David ;

Brodie, Eoln L. ;

Hazen, Terry C. ;

Keller, Martin .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (05) :3291-3301

[2] CYCLIC-AMP IN PROKARYOTES [J].

BOTSFORD, JL ;

HARMAN, JG .

MICROBIOLOGICAL REVIEWS, 1992, 56 (01) :100-122

[3] Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules [J].

Brady, Sean F. .

NATURE PROTOCOLS, 2007, 2 (05) :1297-1305

[4] N-acyl derivatives of arginine and tryptophan isolated from environmental DNA expressed in Escherichia coli [J].

Brady, SF ;

Clardy, J .

ORGANIC LETTERS, 2005, 7 (17) :3613-3616

[5] New natural product families from an environmental DNA (eDNA) gene cluster [J].

Brady, SF ;

Chao, CJ ;

Clardy, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (34) :9968-9969

[6] Long-chain N-acyltyrosine synthases from environmental DNA [J].

Brady, SF ;

Chao, CJ ;

Clardy, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (11) :6865-6870

[7] Long-chain N-acyl amino acid antibiotics isolated from heterologously expressed environmental DNA [J].

Brady, SF ;

Clardy, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (51) :12903-12904

[8] GENETIC CHARACTERIZATION OF MUTATIONS WHICH AFFECT CATABOLITE-SENSITIVE OPERONS IN ESCHERICHIA-COLI, INCLUDING DELETIONS OF GENE FOR ADENYL-CYCLASE [J].

BRICKMAN, E ;

SOLL, L ;

BECKWITH, J .

JOURNAL OF BACTERIOLOGY, 1973, 116 (02) :582-587

[9] Identification of bacteria responsible for ammonia oxidation in freshwater aquaria [J].

Burrell, PC ;

Phalen, CM ;

Hovanec, TA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (12) :5791-5800

[10] Classification and phylogenetic analysis of the cAMP-dependent protein kinase regulatory subunit family [J].

Canaves, JM ;

Taylor, SS .

JOURNAL OF MOLECULAR EVOLUTION, 2002, 54 (01) :17-29