Identification of serum proteins that bind with S100A8, S100A9 and S100A8/A9: Clinical significance of using proteins for monitoring the postoperative condition of liver recipients

被引：4

作者：

Namura, Tomoyo ^{[1
]}

Arai, Satoshi ^{[2
]}

Okawa, Katsuya ^{[3
]}

Koike, Akiko ^{[1
]}

Yamada, Sachiko ^{[1
]}

Saita, Naoko ^{[1
]}

Nagae, Akiko ^{[1
]}

Itoh, Hiroshi ^{[1
]}

Totani, Masayuki ^{[4
]}

Uemoto, Shinji ^{[5
]}

Ikemoto, Masaki ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Kyoto 6068507, Japan

[2] Yamasa Co, Res & Dev Sect, Diagnost Dept, Chiba 2880056, Japan

[3] Kyowa Hakko Kirin Co Ltd, Innovat Drug Res Labs, Gunma 3701295, Japan

[4] Showa Womens Univ, Grad Sch Human Life Sci, Tokyo 1548533, Japan

[5] Kyoto Univ Hosp, Dept Transplantat Unit, Kyoto 6068507, Japan

来源：

CLINICA CHIMICA ACTA | 2010年 / 411卷 / 21-22期

关键词：

S100; proteins; Fibronectin; Inflammation; Neutrophil; Macrophage; CRP; ENDOTHELIAL-CELLS; MRP14; HETERODIMER; FIBRONECTIN; ADHESION; COMPLEX; INDUCE; FAMILY;

D O I：

10.1016/j.cca.2010.07.029

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background Serum proteins that non-specifically bind with human S100A8/A9 (h-S100A8/A9) have been proposed. Our aim was to isolate and identify these proteins, and verify their clinical significance for monitoring the postoperative condition of liver recipients, and further to discuss the transportation of human fibronectin (h-EN) with h-S100A8/A9 and its functional role in vivo. Methods To isolate the serum proteins, recombinant human S100A8. S100A9 and S100A8/A9 affinity columns were used Proteins were identified by mass spectrometry Two enzyme-linked immunosorbent assays (ELISA) were used to measure h-S100A8/A9 and h-FN in the sera of liver recipients Flow cytometry was employed to detect h-S100A8/A9 and h-FN on immunological cells Western blotting was used to confirm serum constituents using antibodies specific to each constituent. Results. One of the proteins was identified with h-FN, and its fluctuation pattern in the serum of the recipient was in contrast to that of CRP Flow cytometry showed a positive reaction for h-S100A8/A9 and h-FN on neutrophils and monocytes, indicating that both proteins exist on these cells Conclusions. The h-FN could be transported with S100A8/A9 in blood and/or on immunological cells, and effectively prevent further attack by various internal oxidants or repair damaged liver tissue 111 VIVO. (C) 2010 Elsevier B V All rights reserved

引用

页码：1766 / 1773

页数：8

共 24 条

[1] S100A9 mediates neutrophil adhesion to fibronectin through activation of β2 integrins [J].