TCL1 as a hub protein associated with the PI3K/AKT signaling pathway in diffuse large B-cell lymphoma based on proteomics methods

被引：5

作者：

Gao, Hai-Xia ^{[1
,2
]}

Li, Si-Jing ^{[1
,2
]}

Niu, Jing ^{[1
,2
]}

Ma, Zhi-Ping ^{[1
]}

Nuerlan, Aijiang ^{[1
,2
]}

Xue, Jing ^{[1
,2
]}

Wang, Meng-Bo ^{[1
,2
]}

Wen-Li Cui ^{[1
]}

Abulajiang, Gulinaer ^{[1
]}

Sang, Wei ^{[1
]}

Zhang, Wei ^{[1
]}

Li, Xin-Xia ^{[1
]}

机构：

[1] Xinjiang Med Univ, Dept Pathol, Affiliated Hosp 1, 137 Liyushan Southern Rd, Urumqi 830054, The Xinjiang Uy, Peoples R China

[2] Xinjiang Med Univ, 393 Xinyi Rd, Urumqi 830011, The Xinjiang Uy, Peoples R China

来源：

PATHOLOGY RESEARCH AND PRACTICE | 2020年 / 216卷 / 02期

基金：

中国国家自然科学基金;

关键词：

DLBCL; iTRAQ; Pathways; TCL1; Clinicopathologic characteristics; Immunotherapeutic target; EPSTEIN-BARR-VIRUS; EXPRESSION; SURVIVAL; ACTIVATION; SUBTYPES; FAMILY;

D O I：

10.1016/j.prp.2019.152799

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

This study aimed to investigate the hub protein related to the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) signaling pathway in diffuse large B-cell lymphoma (DLBCL). We used proteomics methods (iTRAQ) to explore the differentially expressed proteins in the non-germinal center B-cell-like (non-GCB) DLBCL in our previous study. In this study, a total of 137 formalin-fixed paraffin-embedded DLBCL tissue samples were analyzed via immunohistochemistry to verify the expression of TCL1, AKT1 + 2 + 3, IICK beta and to determine the differentially expressed proteins associated with the PI3K/AKT signaling pathway. Spearman correlation was used to analyze the relationship between these proteins, and survival analysis was used to investigate their effects on prognosis. Immunohistochemistry analysis indicated that TCL1, AKT1 + 2 + 3, and IKKI3 were highly positively expressed in DLBCL. Results showed that the expression of TCL1 was related to ethnicity (p = 0.022), primary site (p = 0.045), Ann Arbor stage (p = 0.037), the International Prognostic Index (p = 0.005), beta 2-microglobulin (p = 0.030), BCL2 expression (p < 0.001), and Ki-67 expression (p = 0.008). A positive correlation was found between TCL1 and AKT1 + 2 + 3 (p < 0.001; r = 0.475). A positive correlation was also found between AKT1 + 2 + 3 and IKK beta (p < 0.001; r = 0.342). In survival analysis, anemia, non-treatment with R-CHOP, positive TCL1 expression, and Ki-67 expression >= 50% independently predicted short progression-free survival and overall survival in the total cohort (p < 0.05). Thus, TCL1 as a hub protein is associated with the PI3K/AKT signaling pathway in DLBCL. TCL1 expression indicated a poor prognosis in patients with DLBCL. With further studies, TCL1 may be established as a reliable prognostic biomarker and potential immunotherapeutic target for improving therapeutic efficacy for DLBCL in the future.

引用

页数：11

共 40 条

[21] T cell leukemia 1 oncogene expression depends on the presence of Epstein-Barr virus in the virus-carrying Burkitt lymphoma lines [J].