Physiological and biochemical effects of cadmium toxicity in enzymes involved in nitrogen and amino-acid metabolism in tomato plants

被引：15

作者：

Chaffei-Haouari, Chiraz ^{[1
,2
]}

Carrayol, Elisa ^{[2
]}

Ghorbel, Mohamed Habib ^{[1
]}

Gouia, Houda ^{[1
]}

机构：

[1] Fac Sci Tunis, Dept Sci Biol, Lab Physiol Vegetale, Tunis 1020, Tunisia

[2] INRA, Unite Nutr Azotee Plantes, F-78026 Versailles, France

来源：

ACTA BOTANICA GALLICA | 2009年 / 156卷 / 03期

关键词：

cadmium; glutamine synthetase; glutamate synthase; glutamate dehydrogenase; tomato; NADP-ISOCITRATE DEHYDROGENASE; GLUTAMATE-DEHYDROGENASE; PHOSPHOENOLPYRUVATE CARBOXYLASE; NITRATE ASSIMILATION; SEEDLINGS; PURIFICATION; SYNTHASE; LEAF; REDUCTASE; PATHWAY;

D O I：

10.1080/12538078.2009.10516172

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The activities of several enzymes related to amino-acid metabolism were investigated in tomato plants (Lycopersicon esculentum Mill 63/5F1) in control and treated leaves and roots Glutamine synthetase and glutamate synthese ferredoxine dependent activities rapidly declined in stress condition In presence of cadmium, the decline of glutamine synthetase activity was retarded, while the activity of glutamate synthase significantly and more rapid declined The activity of NADH-dependent glutamate dehydrogenase was markedly increased in presence of cadmium Western blots analysis from cadmium treated plants, showed a decrease in chloroplastic GS (GS2) protein and an increase in cytosolic GS (GS1) and glutamate dehydrogenase (GDH) proteins The activities of phosphoenol pyruvate carboxylase and NADP-dependent isocitrate dehydrogenase also increased in both organs of plantes treated The activity of NAD and NADP-dependent malate dehydrogenase were decreased especially in leaves

引用

页码：477 / 486

页数：10

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