HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)

被引：14

作者：

Ferreres, Federico ^{[2
]}

Pereira, David M. ^{[1
]}

Gil-Izquierdo, Angel ^{[2
]}

Valentao, Patricia ^{[1
]}

Botelho, Joao ^{[1
]}

Mouga, Teresa ^{[3
]}

Andrade, Paula B. ^{[1
]}

机构：

[1] Univ Porto, Fac Pharm, REQUIMTE Dept Pharmacognosy, P-4050047 Oporto, Portugal

[2] CSIC, CEBAS, Res Grp Qual Safety & Bioact Plant Foods, Dept Food Sci & Technol, Murcia, Spain

[3] Polytech Inst Leiria, Sch Tourism & Maritime Technol, GIRM Marine Resources Res Grp, Santuario Sra Remedios, Peniche, Portugal

来源：

JOURNAL OF SEPARATION SCIENCE | 2010年 / 33卷 / 15期

关键词：

Carotenoids; Cytotoxic; HPLC-PAD-atmospheric pressure chemical ionization-MS; Marthasterias glacialis; ASTAXANTHIN;

D O I：

10.1002/jssc.201000197

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea-star). Bio-guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL-2H3 (IC25 = 268 mu g/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC25 = 411 mu g/mL)) cell line, however, toxicity was lower, as it is desired for anti-cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry.

引用

页码：2250 / 2257

页数：8

共 18 条

[1] Britton George., 2004, Carotenoids
[2] Bruneton J., 1999, PHARMACOGNOSIE PHYTO
[3] Chew BP, 1999, ANTICANCER RES, V19, P1849
[4] INVESTIGATIONS OF CAROTENOIDS IN SOME ANIMALS OF ADRIATIC SEA V-ECHINODERMATA
CZECZUGA, B
[J]. HYDROBIOLOGIA, 1977, 54 (02) : 177 - 180
[5] Davies BH., 1976, Chemistry and Biochemistry of Plant Pigments, V2, P38
[6] The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for activities related to reputed anticancer activity
Houghton, Peter
Fang, Rui
Techatanawat, Isariya
Steventon, Glyn
Hylands, Peter J.
Lee, C. C.
[J]. METHODS, 2007, 42 (04) : 377 - 387
[7] Antitumor activity of astaxanthin and its mode of action
Jyonouchi, H
Sun, S
Iijima, K
Gross, MD
[J]. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2000, 36 (01): : 59 - 65
[8] MARINE CAROTENOIDS - RECENT PROGRESS
LIAAENJENSEN, S
[J]. PURE AND APPLIED CHEMISTRY, 1991, 63 (01) : 1 - 12
[9] Carotenoids: Actual knowledge on food sources, intakes, stability and bioavailability and their protective role in humans
Maiani, Giuseppe
Periago Caston, Maria Jesus
Catasta, Giovina
Toti, Elisabetta
Goni Cambrodon, Isabel
Bysted, Anette
Granado-Lorencio, Fernando
Olmedilla-Alonso, Begona
Knuthsen, Pia
Valoti, Massimo
Boehm, Volker
Mayer-Miebach, Esther
Behsnilian, Diana
Schlemmer, Ulrich
[J]. MOLECULAR NUTRITION & FOOD RESEARCH, 2009, 53 : S194 - S218
[10] Characterization of epoxy carotenoids by fast atom bombardment collision-induced dissociation MS/MS
Maoka, T
Fujiwara, Y
Hashimoto, K
Akimoto, N
[J]. LIPIDS, 2004, 39 (02) : 179 - 183

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