Assessment of EGFR mutation status in Tunisian patients with pulmonary adenocarcinoma

被引：8

作者：

Toumi, A. Arfaoui ^{[1
]}

Blel, A. ^{[1
]}

Aloui, R. ^{[1
]}

Zaibi, H. ^{[2
]}

Ksentinini, M. ^{[1
]}

Boudaya, M. S. ^{[3
]}

Znaidi, N. ^{[1
]}

Zidi, Y. ^{[1
]}

Aouina, H. ^{[2
]}

Rommani, S. Rammeh ^{[1
]}

机构：

[1] Tunis El Manar Univ, Charles Nicolle Hosp Tunis, Fac Med Tunis, Dept Pathol, Tunis, Tunisia

[2] Tunis El Manar Univ, Charles Nicolle Hosp Tunis, Fac Med Tunis, Dept Pneumol, Tunis, Tunisia

[3] Tunis El Manar Univ, Charles Nicolle Hosp Tunis, Fac Med Tunis, Dept Surg, Tunis, Tunisia

来源：

CURRENT RESEARCH IN TRANSLATIONAL MEDICINE | 2018年 / 66卷 / 03期

关键词：

EGFR; ALK; Non-small cell lung cancer; Adenocarcinoma; Real-time polymerase chain reaction; CELL LUNG-CANCER; GROWTH-FACTOR-RECEPTOR; NONSMALL CELL; GENE-MUTATIONS; INTERNATIONAL-ASSOCIATION; ACTIVATING MUTATIONS; KRAS MUTATIONS; GEFITINIB; PROGNOSIS; CLASSIFICATION;

D O I：

10.1016/j.retram.2018.02.004

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background. - Despite recent advances, non-small cell lung cancer carries a grim prognosis. For appropriate treatment selection, the updated guidelines recommend broad molecular profiling for all patients with pulmonary adenocarcinoma. Precise histological subtyping and targeted epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK) testing are mandatory. Methods. - Herein, we assessed the EGFR mutation status of 26 formalin fixed-paraffin embedded (FFPE) samples of lung adenocarcinoma. Mutational analysis concerned exons 18-21 of EGFR by real time polymerase chain reaction (Real time-PCR) using the Therascreen EGFR RGQ PCR mutation kit. ALK status was established on 22 among 26 patients using D5F3 antibody with a fully automated Ventana CDx technique. Results. - Activating EGFR mutations were found in 3 men among 26 patients (11.5%). Positive ALK expression was found in 2 cases among 22 patients (9.09%). Conclusion. - Frequency of EGFR mutations in pulmonary adenocarcinomas of our series is similar to that found in the European ones with some particularities. The mutations detected are uncommon. Whereas, we found a high frequency of positive ALK expression in our series compared to frequency reported in literature. Further studies with larger Tunisian series are required to obtain more conclusive results. (C) 2018 Elsevier Masson SAS. All rights reserved.

引用

页码：65 / 70

页数：6