Comparative analysis of the roles of HtrA-like surface proteases in two virulent Staphylococcus aureus strains

被引:54
|
作者
Rigoulay, C [1 ]
Entenza, JM
Halpern, D
Widmer, E
Moreillon, P
Poquet, I
Gruss, A
机构
[1] INRA, Unite Rech Laitieres & Genet Appl, F-78352 Jouy En Josas, France
[2] Inst Fundamental Microbiol, Lausanne, Switzerland
关键词
D O I
10.1128/IAI.73.1.563-572.2005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The HtrA surface protease is involved in the virulence of many pathogens, mainly by its role in stress resistance and bacterial survival. Staphylococcus aureus encodes two putative HtrA-like proteases, referred to as HtrA(1) and HtrA(2). To investigate the roles of HtrA proteins in S. aureus, we constructed htrA(1), htrA(2), and htrA(1) htrA(1) insertion mutants in two genetically different virulent strains, RN6390 and COL. In the RN6390 context, htrA(1) inactivation resulted in sensitivity to puromycin-induced stress. The RN6390 htrA(1) htrA(2) mutant was affected in the expression of several secreted virulence factors comprising the agr regulon. This observation was correlated with the disappearance of the agr RNA III transcript in the RN6390 htrA(1) htrA(2) mutant. The virulence of this mutant was diminished in a rat model of endocarditis. In the COL context, both HtrA(1) and HtrA(2) were essential for thermal stress survival. However, only HtrA, had a slight effect on exoprotein expression. The htrA mutations did not diminish the virulence of the COL strain in the rat model of endocarditis. Our results indicate that HtrA proteins have different roles in S. aureus according to the strain, probably depending on specific differences in the regulation of virulence factor and stress protein expression. We propose that HtrA(1) and HtrA(2) contribute to pathogenicity by controlling the production of certain extracellular factors that are crucial for bacterial dissemination, as revealed in the RN6390 background. We speculate that HtrA proteins act in the agr-dependent regulation pathway by assuring folding and/or maturation of some surface components of the agr system.
引用
收藏
页码:563 / 572
页数:10
相关论文
共 40 条
  • [1] Protective Roles of Folic Acid in the Responses of Bovine Mammary Epithelial Cells to Different Virulent Staphylococcus aureus Strains
    Mi, Siyuan
    Tang, Yongjie
    Shi, Liangyu
    Liu, Xueqin
    Si, Jingfang
    Yao, Yuelin
    Augustino, Serafino M. A.
    Fang, Lingzhao
    Yu, Ying
    BIOLOGY-BASEL, 2021, 10 (11):
  • [2] Multiomics analysis of Staphylococcus aureus ST239 strains resistant to virulent Herelleviridae phages
    Kornienko, M.
    Bespiatykh, D.
    Abdraimova, N.
    Gorodnichev, R.
    Gostev, V.
    Boldyreva, D.
    Selezneva, O.
    Veselovsky, V.
    Pobeguts, O.
    Smirnov, I.
    Arapidi, G.
    Klimina, K.
    Shitikov, E.
    SCIENTIFIC REPORTS, 2024, 14 (01):
  • [3] Joint Genomic and Proteomic Analysis Identifies Meta-Trait Characteristics of Virulent and Non-virulent Staphylococcus aureus Strains
    Bonar, Emilia A.
    Bukowski, Michal
    Hydzik, Marcin
    Jankowska, Urszula
    Kedracka-Krok, Sylwia
    Groborz, Magdalena
    Dubin, Grzegorz
    Akkerboom, Viktoria
    Miedzobrodzki, Jacek
    Sabat, Artur J.
    Friedrich, Alexander W.
    Wladyka, Benedykt
    FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2018, 8
  • [4] Comparative Expression Analysis of Two Thermostable Nuclease Genes in Staphylococcus aureus
    Hu, Yu
    Xie, Yanping
    Tang, Junni
    Shi, Xianming
    FOODBORNE PATHOGENS AND DISEASE, 2012, 9 (03) : 265 - 271
  • [5] Comparative virulence studies and transcriptome analysis of Staphylococcus aureus strains isolated from animals
    Zahid Iqbal
    Mohamed N. Seleem
    Hafiz Iftikhar Hussain
    Lingli Huang
    Haihong Hao
    Zonghui Yuan
    Scientific Reports, 6
  • [6] Comparative virulence studies and transcriptome analysis of Staphylococcus aureus strains isolated from animals
    Iqbal, Zahid
    Seleem, Mohamed N.
    Hussain, Hafiz Iftikhar
    Huang, Lingli
    Hao, Haihong
    Yuan, Zonghui
    SCIENTIFIC REPORTS, 2016, 6
  • [7] Comparative genomics analysis of two Helcococcus kunzii strains co-isolated with Staphylococcus aureus from diabetic foot ulcers
    Durand, Benjamin A. R. N.
    Martinez, Alex Yahiaoui
    Baud, Damien
    Francois, Patrice
    Lavigne, Jean-Philippe
    Dunyach-Remy, Catherine
    GENOMICS, 2022, 114 (03)
  • [8] Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus
    Tibor Botka
    Vladislava Růžičková
    Hana Konečná
    Roman Pantůček
    Ivan Rychlík
    Zbyněk Zdráhal
    Petr Petráš
    Jiří Doškař
    Virus Genes, 2015, 51 : 122 - 131
  • [9] Complete genome analysis of two new bacteriophages isolated from impetigo strains of Staphylococcus aureus
    Botka, Tibor
    Ruzickova, Vladislava
    Konecna, Hana
    Pantucek, Roman
    Rychlik, Ivan
    Zdrahal, Zbynek
    Petras, Petr
    Doskar, Jiri
    VIRUS GENES, 2015, 51 (01) : 122 - 131
  • [10] Comparative genomics of two related ST59 methicillin-resistant Staphylococcus aureus strains in Taiwan
    Hung, W. -C.
    Chen, H. -J.
    Lin, Y. -T.
    Tsai, J. -C.
    Hsueh, P. -R.
    Teng, L. -J.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2013, 42 : S79 - S79