Effects of lentivirus-mediated endostatin on endothelial progenitor cells

被引：5
作者：
Ai, Jing [1 ]
Sun, Jun-Hui [2 ]
Ma, Jian [1 ]
Yao, Ke [1 ]
机构：
[1] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Eye Ctr, Hangzhou 310009, Zhejiang, Peoples R China
[2] Zhejiang Univ, Key Lab Combined Multiorgan Transplantat, Minist Publ Hlth, Affiliated Hosp1,Sch Med, Hangzhou 310003, Zhejiang, Peoples R China
来源：
ONCOTARGET | 2017年 / 8卷 / 55期
基金：
中国国家自然科学基金;
关键词：
endostatin; endothelial progenitor cells; lentiviral vector; gene transfer; cell viability; HEPATOCYTE GROWTH-FACTOR; GENE-THERAPY; CHOROIDAL NEOVASCULARIZATION; TUMOR ANGIOGENESIS; PERIPHERAL-BLOOD; DELIVERY; INHIBITION; VECTORS; ANTIANGIOGENESIS; TRANSPLANTATION;
D O I：
10.18632/oncotarget.21770
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Endothelial progenitor cells (EPCs) are candidates for gene therapies against retinal neovascularization (NV). The aim of present study was to investigate the effects of endostatin transfection on EPC function. In the present study, the EPCs were infected with lentivirus overexpressing endostatin. The transfection effects of endostatin overexpression on the proliferation, migratory, differentiation, apoptosis and the cell cycle of this cell line were determined. The real-time quantitative polymerase chain reaction (RT-qPCR) and western blot assays showed high expression levels of endostatin. A cell counting kit-8 assay showed that endostatin overexpression inhibited EPC proliferation. The transwell assay indicated that endostatin overexpression could suppress EPC migration. Furthermore, endostatin overexpression enhanced apoptosis (as showed by AnnexinV-FITC/propidiumiodide staining analysis), induced differentiation and blocked the cell cycle. As compared with negative control group, EPC viability significantly decreased in gene transfection group. In conclusion, present study determined the feasibility of lentivirus-mediated endostatin gene transfer, and indirectly proved the effect of endostatin secretion on EPCs. Also our study provided a new opportunity for the potential application of gene therapy in retinal NV.
引用
收藏
页码：94431 / 94439
页数：9
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