DNA damage in caged Gammarus fossarum amphipods: A tool for freshwater genotoxicity assessment

被引：55
作者：
Lacaze, Emilie [1 ,2 ]
Devaux, Alain [2 ]
Mons, Raphael [1 ]
Bony, Sylvie [2 ]
Garric, Jeanne [1 ]
Geffard, Alain [3 ]
Geffard, Olivier [1 ]
机构：
[1] Irstea, Unite Rech Milieux Aquat UR MALY, F-69336 Lyon 9, France
[2] Univ Lyon, INRA ENTPE, Lab Sci Environm, F-69518 Vaulx En Velin, France
[3] UFR Sci, URVVC SE EA 2069, Lab Ecotoxicol, F-51687 Reims 2, France
来源：
ENVIRONMENTAL POLLUTION | 2011年 / 159卷 / 06期
关键词：
Gammarus fossarum; Genotoxicity; Caging; Waste water treatment plant effluent; Polymetallic contamination; BIVALVE CORBICULA-FLUMINEA; HEAVY-METAL POLLUTION; GRADIENT RIVER LOT; METALLOTHIONEIN RESPONSE; FIELD TRANSPLANTATION; ZINC BIOACCUMULATION; STRAND BREAKS; CONTAMINATION; CADMIUM; MARINE;
D O I：
10.1016/j.envpol.2011.02.038
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The aim of this study was to propose a tool for freshwater environmental genotoxicity assessment using Gammarus fossarum, a high ecologically relevant species. In a first part, gammarids were caged upstream and downstream wastewater treatment plant effluent output. The sensitivity of genotoxic responses of haemocytes, oocytes and spermatozoa was compared using the Comet assay. Spermatozoa appeared to be the most sensitive, suitable and relevant cell type for genotoxicity risk assessment. In a second part, a watershed-scale study was conducted over 2 years to evaluate the applicability of our caging procedure. The genotoxic impact of a contamination was followed, taking into account seasonal variability. DNA damage in spermatozoa exhibited low basal level and low variability in control upstream sites, providing a reliable discrimination of polluted sites. Finally, DNA damage in caged G. fossarum has been proved to be a sensitive and reproducible tool for freshwater genotoxicity assessment. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1682 / 1691
页数：10
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