Improving the Yield of Blood Cultures from Patients with Early Lyme Disease

被引：36

作者：

Liveris, Dionysios ^{[2
]}

Schwartz, Ira ^{[1
,2
]}

Bittker, Susan ^{[1
]}

Cooper, Denise ^{[1
]}

Iyer, Radha ^{[2
]}

Cox, Mary E. ^{[1
]}

Wormser, Gary P. ^{[1
]}

机构：

[1] New York Med Coll, Div Infect Dis, Dept Med, Valhalla, NY 10595 USA

[2] New York Med Coll, Dept Microbiol & Immunol, Valhalla, NY 10595 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 06期

关键词：

POLYMERASE CHAIN-REACTION; BORRELIA-BURGDORFERI;

D O I：

10.1128/JCM.00350-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Approximately 45% of untreated United States patients with early Lyme disease associated with erythema migrans have a positive blood culture based on microscopic detection of Borrelia burgdorferi in Barbour-Stoenner-Kelly medium after 2 to 12 weeks of incubation. In this study we demonstrate that the yield of blood cultures can be significantly increased to 70.8% by the use of a combined culture-quantitative PCR technique and that among those patients found to have a positive blood culture, positivity was detected in over 90% within just 7 days of incubation. Patients with multiple erythema migrans were almost uniformly culture positive by this technique.

引用

页码：2166 / 2168

页数：3

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