Electrochemical Sensor Based on Oxidation of 2,8-Dihydroxyadenine to Monitor DNA Damage in Calf Thymus DNA

被引：2
作者：
Goyal, Rajendra N. [1 ]
Chatterjee, Sanghamitra [1 ]
Rana, Anoop Raj Singh [1 ]
机构：
[1] Indian Inst Technol, Dept Chem, Roorkee 247667, Uttar Pradesh, India
来源：
ELECTROANALYSIS | 2011年 / 23卷 / 06期
关键词：
Calf thymus DNA; 2,8-Dihydroxyadenine; Edge plane pyrolytic graphite electrode; Single-wall carbon nanotubes; Square wave voltammetry; PERFORMANCE LIQUID-CHROMATOGRAPHY; PYROLYTIC-GRAPHITE ELECTRODE; PURINE METABOLISM; CARBON NANOTUBES; ACID; ADENINE; VOLTAMMETRY; CHEMISTRY; URINE; HPLC;
D O I：
10.1002/elan.201000753
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel and reliable direct electrochemical method has been established to monitor DNA damage in acid hydrolyzed calf thymus DNA, based on the determination of 2,8-dihydroxyadenine (2,8-DHA). A single-wall carbon nanotubes (SWCNT) modified edge plane pyrolytic graphite electrode (EPPGE) has been used as a sensor to monitor the DNA damage. 2,8-DHA the main in vivo adenine oxidation product undergoes oxidation at similar to 395 mV at SWCNT modified EPPGE using square wave voltammetry (SWV). The sensor exhibits potent and persistent electron-mediating behavior. A well-defined oxidation peak for the oxidation of 2,8-DHA was observed at modified electrode with lowering of peak potential and increase in peak current as compared to bare EPPGE. At optimal experimental conditions, the catalytic oxidative peak current was responsive with the 2,8-DHA concentrations ranging from 0.05 nM to 100 nM. The detection limit was 3.8 x 10(-11) M and limit of quantification was 1.27 x 10(-10) M. The modified electrode exhibited high stability and reproducibility.
引用
收藏
页码：1383 / 1390
页数：8
相关论文
共 46 条
  • [1] Acute renal failure in a middle-aged woman with 2,8-dihydroxyadeninuria
    Arnadottir, M
    Laxdal, T
    Hardarson, S
    Asmundsson, P
    [J]. NEPHROLOGY DIALYSIS TRANSPLANTATION, 1997, 12 (09) : 1985 - 1987
  • [2] Carbon nanotubes contain metal impurities which are responsible for the "electrocatalysis" seen at some nanotube-modified electrodes
    Banks, CE
    Crossley, A
    Salter, C
    Wilkins, SJ
    Compton, RG
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (16) : 2533 - 2537
  • [3] BROWN ER, 1964, PHYS METHODS CHEM, P423
  • [4] Recurrence of 2,8-dihydroxyadenine tubulointerstitial lesions in a kidney transplant recipient with a primary presentation of chronic renal failure
    Brown, HA
    [J]. NEPHROLOGY DIALYSIS TRANSPLANTATION, 1998, 13 (04) : 998 - 1000
  • [5] Oxidative damage to DNA: formation, measurement and biochemical features
    Cadet, J
    Douki, T
    Gasparutto, D
    Ravanat, JL
    [J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2003, 531 (1-2) : 5 - 23
  • [6] Trace measurements of plasmid DNAs by adsorptive stripping potentiometry at carbon paste electrodes
    Cai, XH
    Rivas, G
    Farias, PAM
    Shiraishi, H
    Wang, J
    Fojta, M
    Palecek, E
    [J]. BIOELECTROCHEMISTRY AND BIOENERGETICS, 1996, 40 (01): : 41 - 47
  • [7] GOUT, URIC-ACID AND PURINE METABOLISM IN PEDIATRIC NEPHROLOGY
    CAMERON, JS
    MORO, F
    SIMMONDS, HA
    [J]. PEDIATRIC NEPHROLOGY, 1993, 7 (01) : 105 - 118
  • [8] Christian G.D., 1962, JELECTROANAL CHEM, V3, P363
  • [9] 2,8-dihydroxyadenine urolithiasis in a patient with considerable residual adenine phosphoribosyltransferase activity in cell extracts but with mutations in both copies of APRT
    Deng, L
    Yang, M
    Fründ, S
    Wessel, T
    De Abreu, RA
    Tischfield, JA
    Sahota, A
    [J]. MOLECULAR GENETICS AND METABOLISM, 2001, 72 (03) : 260 - 264
  • [10] Electrochemical behaviour of 2,8-dihydroxyadenine at a glassy carbon electrode
    Diculescu, Victor C.
    Piedade, Jose Antonio P.
    Oliveira-Brett, Ana Maria
    [J]. BIOELECTROCHEMISTRY, 2007, 70 (01) : 141 - 146
12345