Preparation, characterization and in vitro release study of carvacrol-loaded chitosan nanoparticles

被引:512
作者
Keawchaoon, Lalita [1 ]
Yoksan, Rangrong [1 ]
机构
[1] Kasetsart Univ, Fac Agroind, Dept Packaging & Mat Technol, Bangkok 10900, Thailand
关键词
Chitosan; Carvacrol; Nanoparticles; Controlled release; Antimicrobial activity; Bioactive compound; DELIVERY SYSTEMS; ESSENTIAL OILS; PROTEIN; BIOAVAILABILITY; MICROPARTICLES; ENCAPSULATION; MICROSPHERES; DERIVATIVES; PARTICLES; CARRIER;
D O I
10.1016/j.colsurfb.2010.12.031
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The fabrication of carvacrol-loaded chitosan nanoparticles was achieved by a two-step method, i.e., oil-in-water emulsion and ionic gelation of chitosan with pentasodium tripolyphosphate. The obtained particles possessed encapsulation efficiency (EE) and loading capacity (LC) in the ranges of 14-31% and 3-21%, respectively, when the initial carvacrol content was 0.25-1.25 g/g of chitosan. The individual particles exhibited a spherical shape with an average diameter of 40-80 nm, and a positively charged surface with a zeta potential value of 25-29 mV. The increment of initial carvacrol content caused a reduction of surface charge. Carvacrol-loaded chitosan nanoparticles showed antimicrobial activity against Staphylococcus aureus, Bacillus cereus and Escherichia coli with an MIC of 0.257 mg/mL. The release of carvacrol from chitosan nanoparticles reached plateau level on day 30, with release amounts of 53% in acetate buffer solution with pH of 3, and 23% and 33% in phosphate buffer solutions with pH of 7 and 11, respectively. The release mechanism followed a Fickian behavior. The release rate was superior in an acidic medium to either alkaline or neutral media, respectively. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:163 / 171
页数:9
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