Determination of thimerosal in pharmaceutical industry effluents and river waters by HPLC coupled to atomic fluorescence spectrometry through post-column UV-assisted vapor generation

被引：22

作者：

Acosta, Gimena ^{[1
,2
]}

Spisso, Adrian ^{[1
,3
]}

Fernandez, Liliana P. ^{[1
,3
]}

Martinez, Luis D. ^{[1
,3
]}

Pacheco, Pablo H. ^{[1
,4
]}

Gil, Raul A. ^{[1
,3
]}

机构：

[1] CCT San Luis, CONICET, Inst Quim San Luis, San Luis, Argentina

[2] Univ Nacl San Luis, Fac Quim Bioquim & Farm, Area Gest Calidad & Salud, San Luis, Argentina

[3] Univ Nacl San Luis, Fac Quim Bioquim & Farm, Area Quim Analit, San Luis, Argentina

[4] Univ Nacl San Luis, Fac Quim Bioquim & Farm, Area Farmacol & Toxicol, San Luis, Argentina

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2015年 / 106卷

关键词：

Thimerosal; Ethylmercury and mercury determination; Pharmaceutical industry effluents; Liquid chromatography; UV-CV-AFS; PERFORMANCE LIQUID-CHROMATOGRAPHY; ORGANIC MERCURY-COMPOUNDS; RP C18 PRECONCENTRATION; EMISSION-SPECTROMETRY; MASS-SPECTROMETRY; INORGANIC MERCURY; ABSORPTION-SPECTROMETRY; METHYLMERCURY; ETHYLMERCURY; SPECIATION;

D O I：

10.1016/j.jpba.2014.09.011

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high performance liquid chromatography coupled with atomic fluorescence spectrometry method for the determination of thimerosal (sodium ethylmercury thiosalicylate, C9H9HgNaO2S), ethylmercury, and inorganic mercury is proposed. Mercury vapor is generated by the post-column reduction of mercury species in formic acid media using UV-radiation. Thimerosal is quantitatively converted to Hg(II) followed by the reduction of Hg(II) to Hg(0). This method is applied to the determination of thimerosal (THM), ethylmercury (EtHg) and inorganic Hg in samples of a pharmaceutical industry effluent, and in waters of the San Luis River situated in the west side of San Luis city (Middle West, Argentine) where the effluents are dumped. The limit of detections, calculated on the basis of the 3 sigma criterion, where 0.09, 0.09 and 0.07 mu g L-1 for THM, EtHg(II) and for Hg(II), respectively. Linearity was attained from levels close to the detection limit up to at least 100 mu g L-1. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：79 / 84

页数：6

共 29 条

[1] An assessment of thimerosal use in childhood vaccines [J].