Contribution of SARS-CoV-2 RNA shedding routes to RNA loads in wastewater

被引:65
作者
Crank, K. [1 ]
Chen, W. [1 ]
Bivins, A. [1 ]
Lowry, S. [2 ]
Bibby, K. [1 ]
机构
[1] Univ Notre Dame, Dept Civil & Environm Engn & Earth Sci, 156 Fitzpatrick Hall, South Bend, IN 46556 USA
[2] Georgia Inst Technol, Dept Civil & Environm Engn, Atlanta, GA 30332 USA
基金
美国国家科学基金会;
关键词
SARS-CoV-2; RNA; Shedding route; Wastewater; Wastewater surveillance; Wastewater-based epidemiology; COVID-19; SETTLED SOLIDS; COVID-19;
D O I
10.1016/j.scitotenv.2021.150376
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A portion of those infected with SARS-CoV-2 shed the virus and its genetic material in respiratory fluids, saliva, urine, and stool, thus giving the potential to monitor for infections via wastewater. Wastewater surveillance efforts to date have largely assumed that stool shedding has been the primary source of SARS-CoV-2 RNA signal; however, there are increasing questions about the possible contribution of other shedding routes, with implica-tions for wastewater surveillance design and feasibility. In this study we used clinical SARS-CoV-2 RNA shedding data and a Monte Carlo framework to assess the relative contribution of various shedding routes on SARS-CoV-2 RNA loads in wastewater. Stool shedding dominated total SARS-CoV-2 RNA load for community-level surveil-lance, with mean contributions more than two orders of magnitude greater than other shedding routes. How-ever, RNA loads were more nuanced when considering building-level monitoring efforts designed to identify a single infected individual, where any shedding route could plausibly contribute a detectable signal. The greatest source of model variability was viral load in excreta, suggesting that future modeling efforts may be improved by incorporating specific modeling scenarios with precise SARS-CoV-2 shedding data, and beyond that wastewater surveillance must continue to account for large variability during data analysis and reporting. Importantly, the findings imply that wastewater surveillance at finer spatial scales is not entirely dependent on shedding via feces for sensitive detection of infections thus enlarging the potential use cases of wastewater as a non-intrusive surveillance methodology. (c) 2021 Elsevier B.V. All rights reserved.
引用
收藏
页数:6
相关论文
共 50 条
  • [31] Monitoring of SARS-CoV-2 RNA in wastewater as an epidemiological surveillance tool in Mendoza, Argentina
    Giraud-Billoud, Maximiliano
    Cuervo, Paula
    Altamirano, Jorgelina C.
    Pizarro, Marcela
    Aranibar, Julieta N.
    Catapano, Adolfo
    Cuello, Hector
    Masachessi, Gisela
    Vega, Israel A.
    SCIENCE OF THE TOTAL ENVIRONMENT, 2021, 796
  • [32] SARS-CoV-2 RNA abundance in wastewater as a function of distinct urban sewershed size
    Holm, Rochelle H.
    Mukherjee, Anish
    Rai, Jayesh P.
    Yeager, Ray A.
    Talley, Daymond
    Rai, Shesh N.
    Bhatnagar, Aruni
    Smith, Ted
    ENVIRONMENTAL SCIENCE-WATER RESEARCH & TECHNOLOGY, 2022, 8 (04) : 807 - 819
  • [33] Detection of SARS-CoV-2 RNA in wastewater, river water, and hospital wastewater of Nepal
    Tandukar, Sarmila
    Sthapit, Niva
    Thakali, Ocean
    Malla, Bikash
    Sherchan, Samendra P.
    Shakya, Bijay Man
    Shrestha, Laxman P.
    Sherchand, Jeevan B.
    Joshi, Dev Raj
    Lama, Bhupendra
    Haramoto, Eiji
    SCIENCE OF THE TOTAL ENVIRONMENT, 2022, 824
  • [34] Variant-specific SARS-CoV-2 shedding rates in wastewater
    Prasek, Sarah M.
    Pepper, Ian L.
    Innes, Gabriel K.
    Slinski, Stephanie
    Betancourt, Walter Q.
    Foster, Aidan R.
    Yaglom, Hayley D.
    Porter, W. Tanner
    Engelthaler, David M.
    Schmitz, Bradley W.
    SCIENCE OF THE TOTAL ENVIRONMENT, 2023, 857
  • [35] Upper respiratory tract SARS-CoV-2 RNA loads in symptomatic and asymptomatic children and adults
    Costa, Rosa
    Bueno, Felipe
    Albert, Eliseo
    Torres, Ignacio
    Carbonell-Sahuquillo, Silvia
    Barres-Fernandez, Ana
    Sanchez, David
    Padron, Carmelo
    Colomina, Javier
    Carreno, Maria Isabel Lazaro
    Breton-Martinez, Jose Rafael
    Martinez-Costa, Cecilia
    Navarro, David
    CLINICAL MICROBIOLOGY AND INFECTION, 2021, 27 (12) : 1858.e1 - 1858.e7
  • [36] Decay of RNA and infectious SARS-CoV-2 and murine hepatitis virus in wastewater
    Purves, Kevin
    Reynolds, Liam J.
    Sala-Comorera, Laura
    Martin, Niamh A.
    Dahly, Darren L.
    Meijer, Wim G.
    Fletcher, Nicola F.
    SCIENCE OF THE TOTAL ENVIRONMENT, 2024, 944
  • [37] SARS-CoV-2 RNA Is Readily Detectable at Least 8 Months after Shedding in an Isolation Facility
    Coil, David A.
    Pechacek, Randi
    Kaze, Mo
    Zuniga-Montanez, Rogelio
    Guerrero, Roque G.
    Eisen, Jonathan A.
    Shapiro, Karen
    Bischel, Heather N.
    MSPHERE, 2022, 7 (06)
  • [38] Nationwide Trends in COVID-19 Cases and SARS-CoV-2 RNA Wastewater Concentrations in the United States
    Duvallet, Claire
    Wu, Fuqing
    McElroy, Kyle A.
    Imakaev, Maxim
    Endo, Noriko
    Xiao, Amy
    Zhang, Jianbo
    Floyd-O'Sullivan, Roisin
    Powell, Morgan M.
    Mendola, Samuel
    Wilson, Shane T.
    Cruz, Francis
    Melman, Tamar
    Sathyanarayana, Chaithra Lakshmi
    Olesen, Scott W.
    Erickson, Timothy B.
    Ghaeli, Newsha
    Chai, Peter
    Alm, Eric J.
    Matus, Mariana
    ACS ES&T WATER, 2022, : 1899 - 1909
  • [39] An efficient method to enhance recovery and detection of SARS-CoV-2 RNA in wastewater
    Kumblathan, Teresa
    Liu, Yanming
    Qiu, Yuanyuan
    Pang, Lilly
    Hrudey, Steve E.
    Le, X. Chris
    Li, Xing -Fang
    JOURNAL OF ENVIRONMENTAL SCIENCES, 2023, 130 : 139 - 148
  • [40] Impact of sewer biofilms on fate of SARS-CoV-2 RNA and wastewater surveillance
    Li, Jiaying
    Ahmed, Warish
    Metcalfe, Suzanne
    Smith, Wendy J. M.
    Choi, Phil M.
    Jackson, Greg
    Cen, Xiaotong
    Zheng, Min
    Simpson, Stuart L.
    Thomas, Kevin V.
    Mueller, Jochen F.
    Thai, Phong K.
    NATURE WATER, 2023, 1 (03): : 272 - 280