Translocation of Intratracheally Instilled Multiwall Carbon Nanotubes to Lung-Associated Lymph Nodes in Rats

被引：24

作者：

Aiso, Shigetoshi ^{[1
]}

Kubota, Hisayo ^{[2
]}

Umeda, Yumi ^{[1
]}

Kasai, Tatsuya ^{[1
]}

Takaya, Mitsutoshi ^{[2
]}

Yamazaki, Kazunori ^{[1
]}

Nagano, Kasuke ^{[1
]}

Sakai, Toshio ^{[3
]}

Koda, Shigeki ^{[2
]}

Fukushima, Shoji ^{[1
]}

机构：

[1] Japan Ind Safety & Hlth Assoc, Japan Bioassay Res Ctr, Kanagawa 2570015, Japan

[2] NIOSH, Tama Ku, Kawasaki, Kanagawa 2148585, Japan

[3] SAKAI Electron Microscopy Applicat Lab, Minuma Ku, Saitama 3370033, Japan

来源：

INDUSTRIAL HEALTH | 2011年 / 49卷 / 02期

关键词：

Multiwall carbon nanotube; Lung-associated lymph node; Mediastinal lymph node; Macrophages; Granuloma; PULMONARY; TOXICITY; FIBERS; MOUSE; MICE;

D O I：

10.2486/indhealth.MS1213

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In order to assess the extrapulmonary effects of multiwall carbon nanotubes (MWCNT), deposition of MWCNT and histopathologic changes in lung-associated lymph nodes (LALN) were examined in MWCNT-administered rats. At the age of 13 wk, male F344 rats were intratracheally instilled with MWCNT at a dose of 0 (vehicle). 40 or 160 mu g/rat. The rats were sacrificed on Day I. 7, 28 or 91 after instillation and light microscopic examinations were performed on LALN tissues. MWCNT was translocated to right and left posterior mediastinal lymph nodes and parathymic lymph nodes. Deposition of MWCNT was greater in the posterior mediastinal lymph node than in the parathymic lymph node, and the amount of MWCNT deposited in these two lymph nodes increased gradually and dose-dependently with time. MWCNT was phagocytosed by nodal macrophages, and some of the MWCNT-laden macrophages were aggregated. Transmission electron microscopic (TEM) observation confirmed the presence of MWCNT fibers with a characteristic multi-walled cylindrical structure.

引用

页码：215 / 220

页数：6

共 15 条

[1] Pulmonary Toxicity of Intratracheally Instilled Multiwall Carbon Nanotubes in Male Fischer 344 Rats
Aiso, Shigetoshi
Yamazaki, Kazunori
Umeda, Yumi
Asakura, Masumi
Kasai, Tatsuya
Takaya, Mitsutoshi
Toya, Tadao
Koda, Shigeki
Nagano, Kasuke
Arito, Heihachiro
Fukushima, Shoji
[J]. INDUSTRIAL HEALTH, 2010, 48 (06) : 783 - 795
[2] [Anonymous], 1996, GUID CAR US LAB AN
[3] Inhalation Toxicity of Multiwall Carbon Nanotubes in Rats Exposed for 3 Months
Ma-Hock, Lan
Treumann, Silke
Strauss, Volker
Brill, Sandra
Luizi, Frederic
Mertler, Michael
Wiench, Karin
Gamer, Armin O.
van Ravenzwaay, Bennard
Landsiedel, Robert
[J]. TOXICOLOGICAL SCIENCES, 2009, 112 (02) : 468 - 481
[4] Mitchell LA, 2009, NAT NANOTECHNOL, V4, P451, DOI [10.1038/nnano.2009.151, 10.1038/NNANO.2009.151]
[5] Mitchell LA, 2007, TOXICOL SCI, V100, P203, DOI 10.1093/toxsci/kfm196
[6] MORROW PE, 1972, ANN NY ACAD SCI, V22, P46
[7] Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot study
Poland, Craig A.
Duffin, Rodger
Kinloch, Ian
Maynard, Andrew
Wallace, William A. H.
Seaton, Anthony
Stone, Vicki
Brown, Simon
MacNee, William
Donaldson, Ken
[J]. NATURE NANOTECHNOLOGY, 2008, 3 (07) : 423 - 428
[8] Mouse pulmonary dose- and time course-responses induced by exposure to multi-walled carbon nanotubes
Porter, Dale W.
Hubbs, Ann F.
Mercer, Robert R.
Wu, Nianqiang
Wolfarth, Michael G.
Sriram, Krishnan
Leonard, Stephen
Battelli, Lori
Schwegler-Berry, Diane
Friend, Sherry
Andrew, Michael
Chen, Bean T.
Tsuruoka, Shuji
Endo, Morinobu
Castranova, Vincent
[J]. TOXICOLOGY, 2010, 269 (2-3) : 136 - 147
[9] CELLULAR AND MOLECULAR-BASIS OF THE ASBESTOS-RELATED DISEASES
ROM, WN
TRAVIS, WD
BRODY, AR
[J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1991, 143 (02): : 408 - 422
[10] LONG-TERM RETENTION AND CLEARANCE OF PARTICLES INHALED BY MAMMALIAN-SPECIES
SNIPES, MB
[J]. CRITICAL REVIEWS IN TOXICOLOGY, 1989, 20 (03) : 175 - 211

← 1 2 →